Correlation between the Effects of Polyamine Analogues on DNA Conformation and Cell Growth

Hirak S. Basu, Dennis F. Deen, Warren P. Lubich, Laurence J. Marton, Burt G. Feuerstein, Raymond J. Bergeron, Keijiro Samejima

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Effects of a number of synthetic analogues of the natural polyamines on the B-Z transition of poly(dG-me5dC) and on the aggregation of calf thymus DNA in solution were studied using circular dichroic and UV spectroscopy. The efficiency of induction of the B-Z transition decreased with a decrease in the length of the central alkyl chain of the analogues, and the ability of analogues to aggregate DNA was markedly reduced for compounds ethylated at the terminal amines. Both structural variations appear to have important effects on the biological functions of polyamines. Most analogues studied depleted intracellular levels of natural polyamines, but only those that did not readily induce the B-Z transition and/or aggregate DNA were good inhibitors of cell growth. All but one of the analogues studied were able to rescue cells–at least in part–from the growth-inhibitory effects of a-difluoromethylornithine. The single analogue that was unable to effect rescue also failed to induce both the B-Z transition and the aggregation of DNA.

Original languageEnglish (US)
Pages (from-to)5591-5597
Number of pages7
JournalCancer Research
Volume49
Issue number20
StatePublished - Oct 15 1989

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Correlation between the Effects of Polyamine Analogues on DNA Conformation and Cell Growth'. Together they form a unique fingerprint.

  • Cite this

    Basu, H. S., Deen, D. F., Lubich, W. P., Marton, L. J., Feuerstein, B. G., Bergeron, R. J., & Samejima, K. (1989). Correlation between the Effects of Polyamine Analogues on DNA Conformation and Cell Growth. Cancer Research, 49(20), 5591-5597.