Correlation of freeze-fracture and scanning electron microscopy of epiphyseal chondrocytes

Thomas K. Borg, Raymond B Runyan, Roy E. Wuthier

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Chondrocytes in epiphyseal cartilage were examined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) using freeze-fracture techniques. Freeze-fracture replicas showed large numbers of fingerlike, 0.11-0.15 μm diameter, projections from the chondrocyte surface, with numerous 95-180 Å diameter intramembranous particles associated with both the cell membrane surface and these projections. With SEM, these cytoplasmic projections were also obvious, but appeared collapsed into clusters of globular-shaped projections on the surface of the chondrocytes. With freeze-fracture techniques, in which shrinkage artifacts were essentially eliminated, the cytoplasmic projections were often seen in intimate contact with the extracapsular matrix. However, with chondrocytes prepared by both SEM and conventional TEM, there was evidence of shrinkage, the cytoplasmic projections having little contact with the extracapsular matrix. These findings show that the cytoplasmic processes are not artifacts of tissue processing and provide morphological evidence in support of the hypothesis that matrix vesicles are of cellular origin.

Original languageEnglish (US)
Pages (from-to)237-241
Number of pages5
JournalCalcified Tissue Research
Volume26
Issue number1
DOIs
StatePublished - Dec 1978
Externally publishedYes

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Chondrocytes
Electron Scanning Microscopy
Cell Surface Extensions
Transmission Electron Microscopy
Artifacts
Growth Plate

Keywords

  • Cell processes
  • Epiphyseal chondrocytes
  • Freezefracture
  • Membrane particles
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Endocrinology

Cite this

Correlation of freeze-fracture and scanning electron microscopy of epiphyseal chondrocytes. / Borg, Thomas K.; Runyan, Raymond B; Wuthier, Roy E.

In: Calcified Tissue Research, Vol. 26, No. 1, 12.1978, p. 237-241.

Research output: Contribution to journalArticle

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