Correlation of human decidual and cord blood mononuclear cell cytokine production

Mark A Brown, Monica Gustafson, Sandra Saldãna, Ali Baradaran, Hugh Miller, Marilyn Halonen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Human decidua has been shown to produce a number of cytokines. We hypothesized that decidual cytokine production influences cord blood mononuclear cell (CBMC) cytokine production and that cytokine profiles of decidua from allergic women differ from those of decidua from nonallergic women. Using enzyme-linked immunosorbent assay, we measured unstimulated and concanavalin A/phorbol myristate acetate-stimulated production of interleukin-4 (IL-4), IL-5, IL-10, IL-13 and interferon- γ (IFN-γ) by decidual explants from 59 healthy women delivered by unlabored cesarean section and from corresponding CBMCs in 39 of the 59. Except for IL-10, there was little or no unstimulated cytokine production. There was a strong correlation between stimulated decidual and stimulated CBMC IFN-γ production (p = 0.01). In allergic women the ratio of IL-13 to IL-4 production was increased in stimulated explants (p = 0.03). Stimulated CBMCs from infants of allergic mothers were more likely to produce detectable levels of IL-5 than those from infants of nonallergic mothers (p = 0.04) and had a tendency toward higher IL-13 levels as well (p = 0.07). These results suggest that maternal and fetal IFN-γ production is closely linked and that maternal allergy appears to influence cytokine production in the neonate for IL-5 and possibly IL-13.

Original languageEnglish (US)
Pages (from-to)1336-1343
Number of pages8
JournalHuman Immunology
Volume65
Issue number11
DOIs
StatePublished - Nov 2004
Externally publishedYes

Fingerprint

Fetal Blood
Blood Cells
Interleukin-13
Cytokines
Decidua
Interleukin-5
Mothers
Interferons
Interleukin-4
Interleukin-10
Tetradecanoylphorbol Acetate
Concanavalin A
Cesarean Section
Hypersensitivity
Enzyme-Linked Immunosorbent Assay
Newborn Infant

Keywords

  • allergy
  • cord blood
  • cytokines
  • deciduas

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Brown, M. A., Gustafson, M., Saldãna, S., Baradaran, A., Miller, H., & Halonen, M. (2004). Correlation of human decidual and cord blood mononuclear cell cytokine production. Human Immunology, 65(11), 1336-1343. https://doi.org/10.1016/j.humimm.2004.08.176

Correlation of human decidual and cord blood mononuclear cell cytokine production. / Brown, Mark A; Gustafson, Monica; Saldãna, Sandra; Baradaran, Ali; Miller, Hugh; Halonen, Marilyn.

In: Human Immunology, Vol. 65, No. 11, 11.2004, p. 1336-1343.

Research output: Contribution to journalArticle

Brown, MA, Gustafson, M, Saldãna, S, Baradaran, A, Miller, H & Halonen, M 2004, 'Correlation of human decidual and cord blood mononuclear cell cytokine production', Human Immunology, vol. 65, no. 11, pp. 1336-1343. https://doi.org/10.1016/j.humimm.2004.08.176
Brown, Mark A ; Gustafson, Monica ; Saldãna, Sandra ; Baradaran, Ali ; Miller, Hugh ; Halonen, Marilyn. / Correlation of human decidual and cord blood mononuclear cell cytokine production. In: Human Immunology. 2004 ; Vol. 65, No. 11. pp. 1336-1343.
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