Cotranslational integration of soybean (Glycine max) oil body membrane protein oleosin into microsomal membranes

Deborah S. Loer, Eliot M. Herman

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

Storage triglycerides in oil seeds are sequestered in discrete organelles termed oil bodies. They are bounded by a monolayer of phospholipids in which a few distinct proteins (oleosins) are embedded. Synthesis of soybean (Glycine max) 24-kD oleosin was analyzed by in vitro transcription and translation in reticulocyte lysate in the presence of canine microsomes. Our results show that 24-kD oleosin is cotranslationally integrated into microsomal membranes. We demonstrate that oleosin is integrated into a bilayer membrane in preference to the oil body monolayer membrane, indicating that oleosin is synthesized on the endoplasmic reticulum (ER). A new model of oil body assembly involving a conformational change through initial association with the ER membrane is proposed.

Original languageEnglish (US)
Pages (from-to)993-998
Number of pages6
JournalPlant physiology
Volume101
Issue number3
DOIs
StatePublished - Mar 1993

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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