Cotton leaf curl Gezira virus-satellite DNAs represent a divergent, geographically isolated Nile Basin lineage: Predictive identification of a satDNA REP-binding motif

A. M. Idris, Rob W. Briddon, Simon E. Bull, Judith K Brown

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Cotton leaf curl Gezira virus (CLCuGV), a species of the genus Begomovirus (family Geminiviridae), was recently cloned from cotton, okra, and Sida alba plants exhibiting leaf-curling and vein-thickening symptoms in Sudan. Here, we describe a previously unknown lineage of single-stranded DNA satellite (satDNA) molecules, which are associated with CLCuGV, and are required for development of characteristic disease symptoms. Co-inoculation of cotton and Nicotiana benthamiana plants with satDNAs cloned from cotton, okra, and S. alba, together with CLCuGV as the 'helper virus' resulted in the development of characteristic leaf-curling and vein-thickening symptoms in both hosts. An anatomical study of symptomatic, virus-infected cotton leaves revealed that spongy parenchyma cells had developed instead of collenchyma cells at the sites of vein thickening. Phylogenetically, the CLCuGV-associated satDNAs from Sudan, together with their closest relatives from Egypt, form a new satDNA lineage comprising only satDNAs from the Upper and Lower Nile Basins. Analysis of satellites and their helper virus sequences identified a predicted REP-binding site consisting of the directly repeated sequence, 'CGGTACTCA', and an inverted repeated sequence, 'TGAGTACCG', which occur in the context of a 17-nucleotide motif. The conserved REP-binding motif identified herein, together with strict geographic isolation, and apparent host-restriction, may be the collective hallmark of these new satDNA-begomovirus lineages, extant in the Nile Basin.

Original languageEnglish (US)
Pages (from-to)19-32
Number of pages14
JournalVirus Research
Volume109
Issue number1
DOIs
StatePublished - Apr 2005

Fingerprint

Satellite DNA
Viruses
Begomovirus
Abelmoschus
Helper Viruses
Veins
Sudan
Malvaceae
Geminiviridae
Sequence Inversion
Nucleotide Motifs
Plant Leaves
Egypt
Single-Stranded DNA
Tobacco
Binding Sites

Keywords

  • Begomovirus
  • Cotton virus
  • DNA β
  • DNA satellite
  • Geminiviridae
  • Helper virus

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

Cotton leaf curl Gezira virus-satellite DNAs represent a divergent, geographically isolated Nile Basin lineage : Predictive identification of a satDNA REP-binding motif. / Idris, A. M.; Briddon, Rob W.; Bull, Simon E.; Brown, Judith K.

In: Virus Research, Vol. 109, No. 1, 04.2005, p. 19-32.

Research output: Contribution to journalArticle

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