Covalent coupling of bovine growth hormone to its receptor in bovine liver membranes

Lokenga Badinga, Robert J Collier, William W. Thatcher, Sergio J. Quintana, Fuller W. Bazer

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The structure of bovine somatotropin receptor was examined following covalent coupling of iodinated recombinant bovine growth hormone ([125I]rbGH) to bovine liver membrane receptors using ethylene glycol bis(succinimidyl succinate). Iodinated rbGH was incorporated into a complex of estimated Mr of 140000 under reducing conditions. Excess unlabeled rbGH, but not bovine prolactin (bPRL), inhibited completely the incorporation of [125I]rbGH into the Mr = 140000 species. In dairy bulls, the Mr = 140000 complex was undetectable soon after birth but became predominant at 6 months of age. No evidence was found to support presence of bPRL receptors in steer liver membranes. Assuming a 1:1 stoichiometry of hormone binding to receptor, it appears that bGH binds to a major receptor subunit of Mr = 119000 which does not recognize bPRL.

Original languageEnglish (US)
Pages (from-to)85-89
Number of pages5
JournalMolecular and Cellular Endocrinology
Volume52
Issue number1-2
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Prolactin
Liver
Prolactin Receptors
Membranes
Somatotropin Receptors
Dairies
Stoichiometry
Hormones
Growth Hormone
Parturition
bovine growth hormone
ethylene glycolylbis(succinimidyl succinate)

Keywords

  • Bovine growth hormone
  • Cross-linking
  • Somatotropin receptor

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Covalent coupling of bovine growth hormone to its receptor in bovine liver membranes. / Badinga, Lokenga; Collier, Robert J; Thatcher, William W.; Quintana, Sergio J.; Bazer, Fuller W.

In: Molecular and Cellular Endocrinology, Vol. 52, No. 1-2, 1987, p. 85-89.

Research output: Contribution to journalArticle

Badinga, Lokenga ; Collier, Robert J ; Thatcher, William W. ; Quintana, Sergio J. ; Bazer, Fuller W. / Covalent coupling of bovine growth hormone to its receptor in bovine liver membranes. In: Molecular and Cellular Endocrinology. 1987 ; Vol. 52, No. 1-2. pp. 85-89.
@article{65416da2f1e348d883c9026ed74efec0,
title = "Covalent coupling of bovine growth hormone to its receptor in bovine liver membranes",
abstract = "The structure of bovine somatotropin receptor was examined following covalent coupling of iodinated recombinant bovine growth hormone ([125I]rbGH) to bovine liver membrane receptors using ethylene glycol bis(succinimidyl succinate). Iodinated rbGH was incorporated into a complex of estimated Mr of 140000 under reducing conditions. Excess unlabeled rbGH, but not bovine prolactin (bPRL), inhibited completely the incorporation of [125I]rbGH into the Mr = 140000 species. In dairy bulls, the Mr = 140000 complex was undetectable soon after birth but became predominant at 6 months of age. No evidence was found to support presence of bPRL receptors in steer liver membranes. Assuming a 1:1 stoichiometry of hormone binding to receptor, it appears that bGH binds to a major receptor subunit of Mr = 119000 which does not recognize bPRL.",
keywords = "Bovine growth hormone, Cross-linking, Somatotropin receptor",
author = "Lokenga Badinga and Collier, {Robert J} and Thatcher, {William W.} and Quintana, {Sergio J.} and Bazer, {Fuller W.}",
year = "1987",
doi = "10.1016/0303-7207(87)90100-6",
language = "English (US)",
volume = "52",
pages = "85--89",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

TY - JOUR

T1 - Covalent coupling of bovine growth hormone to its receptor in bovine liver membranes

AU - Badinga, Lokenga

AU - Collier, Robert J

AU - Thatcher, William W.

AU - Quintana, Sergio J.

AU - Bazer, Fuller W.

PY - 1987

Y1 - 1987

N2 - The structure of bovine somatotropin receptor was examined following covalent coupling of iodinated recombinant bovine growth hormone ([125I]rbGH) to bovine liver membrane receptors using ethylene glycol bis(succinimidyl succinate). Iodinated rbGH was incorporated into a complex of estimated Mr of 140000 under reducing conditions. Excess unlabeled rbGH, but not bovine prolactin (bPRL), inhibited completely the incorporation of [125I]rbGH into the Mr = 140000 species. In dairy bulls, the Mr = 140000 complex was undetectable soon after birth but became predominant at 6 months of age. No evidence was found to support presence of bPRL receptors in steer liver membranes. Assuming a 1:1 stoichiometry of hormone binding to receptor, it appears that bGH binds to a major receptor subunit of Mr = 119000 which does not recognize bPRL.

AB - The structure of bovine somatotropin receptor was examined following covalent coupling of iodinated recombinant bovine growth hormone ([125I]rbGH) to bovine liver membrane receptors using ethylene glycol bis(succinimidyl succinate). Iodinated rbGH was incorporated into a complex of estimated Mr of 140000 under reducing conditions. Excess unlabeled rbGH, but not bovine prolactin (bPRL), inhibited completely the incorporation of [125I]rbGH into the Mr = 140000 species. In dairy bulls, the Mr = 140000 complex was undetectable soon after birth but became predominant at 6 months of age. No evidence was found to support presence of bPRL receptors in steer liver membranes. Assuming a 1:1 stoichiometry of hormone binding to receptor, it appears that bGH binds to a major receptor subunit of Mr = 119000 which does not recognize bPRL.

KW - Bovine growth hormone

KW - Cross-linking

KW - Somatotropin receptor

UR - http://www.scopus.com/inward/record.url?scp=0023185045&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023185045&partnerID=8YFLogxK

U2 - 10.1016/0303-7207(87)90100-6

DO - 10.1016/0303-7207(87)90100-6

M3 - Article

C2 - 3622922

AN - SCOPUS:0023185045

VL - 52

SP - 85

EP - 89

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

IS - 1-2

ER -