Creating a conditional mutation of Wnt‐1 by antisense transgenesis provides evidence that Wnt‐1 is not essential for spermatogenesis

Robert P. Erickson, Li‐Wen ‐W Lai, Judy Grimes

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

We have used mice transgenic for an antisense construct for Wnt‐1 to study the role of this gene in post‐meiotic sperm development. The human PGK‐2 promoter provided levels of Wnt‐1 antisense mRNA in testes in 5 transgenic lines greatly in excess of Wnt‐1 mRNA concentrations, and Wnt‐1 mRNA levels were greatly decreased in the lines, by 98% in three of them. There was a general correlation between copy number of the insert, levels of antisense RNA, and decreases in mRNA. There was little effect of the antisense transgene on fertility or testicular histology suggesting that normal levels of Wnt‐ 1 transcript are not essential for spermatogenesis. © 1993Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)274-281
Number of pages8
JournalDevelopmental Genetics
Volume14
Issue number4
DOIs
StatePublished - 1993

Keywords

  • Transgenesis
  • antisense RNA
  • phosphoglycerate kinase 2 promoter
  • spermatogenesis
  • wingless

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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