Critical role of the transient activation of p38 MAPK in the etiology of skeletal muscle insulin resistance induced by low-level in vitro oxidant stress

Maggie K. Diamond-Stanic, Elizabeth M. Marchionne, Mary K. Teachey, David E. Durazo, John S. Kim, Erik J. Henriksen

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31 Scopus citations

Abstract

Increased cellular exposure to oxidants may contribute to the development of insulin resistance and type 2 diabetes. Skeletal muscle is the primary site of insulin-dependent glucose disposal in the body; however, the effects of oxidative stress on insulin signaling and glucose transport activity in mammalian skeletal muscle are not well understood. We therefore studied the effects of a low-level in vitro oxidant stress (30-40μM H2O2) on basal and insulin-stimulated (5mU/ml) glucose transport activity and insulin signaling at 2, 4, and 6h in isolated rat soleus muscle. H2O2 increased basal glucose transport activity at 2 and 4h, but not at 6h. This low-level oxidant stress significantly impaired insulin-stimulated glucose transport activity at all time points, and was associated with inhibition of insulin-stimulated phosphorylation of Akt Ser473 and GSK-3β Ser9. In the presence of insulin, H2O2 decreased total protein expression of IRS-1 at 6h and IRS-2 at 4 and 6h. Phosphorylation of p38 MAPK Thr180/Tyr182 was transiently increased by H2O2 in the presence and absence of insulin at 2 and 4h, but not at 6h. Selective inhibition of p38 MAPK with A304000 partially rescued the H2O2-induced reduction in insulin-stimulated glucose transport activity. These results indicate that direct in vitro exposure of isolated mammalian skeletal muscle to a low-level oxidant stress impairs distal insulin signaling and insulin-stimulated glucose transport activity, at least in part, due to a p38 MAPK-dependent mechanism.

Original languageEnglish (US)
Pages (from-to)439-444
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume405
Issue number3
DOIs
StatePublished - Feb 18 2011

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Keywords

  • Glucose transport
  • Hydrogen peroxide
  • Insulin signaling
  • Soleus muscle

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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