Cross-clade human immunodeficiency virus (HIV)-specific cytotoxic T- lymphocyte responses in HIV-infected Zambians

M. R. Betts, J. Krowka, C. Santamaria, K. Balsamo, F. Gao, G. Mulundu, C. Luo, N. N'Gandu, H. Sheppard, B. H. Hahn, S. Allen, Jeffrey A Frelinger

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Abstract

We have examined cross-clade HIV-specific cytotoxic T-lymphocyte (CTL) activity in peripheral blood of eight Zambian individuals infected with non- B-clade human immunodeficiency virus type 1 (HIV-1). Heteroduplex mobility assay and partial sequence analysis of env and gag genes strongly suggests that all the HIV-infected subjects were infected with clade C HIV-1. Six of eight C-clade HIV-infected individuals elicited CTL activity specific for recombinant vaccinia virus-infected autologous targets expressing HIV gag- pol-env derived from B-clade HIV-1 (IIIB). Recognition of individual recombinant HIV-1 B-clade vaccinia virus-infected targets expressing gag, pol, or env was variable among the patients tested, indicating that cross- clade CTL activity is not limited to a single HIV protein. These data demonstrate that HIV clade C-infected individuals can mount vigorous HIV clade B-reactive CTL responses.

Original languageEnglish (US)
Pages (from-to)8908-8911
Number of pages4
JournalJournal of Virology
Volume71
Issue number11
StatePublished - 1997
Externally publishedYes

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cytotoxic T-lymphocytes
Human immunodeficiency virus
Cytotoxic T-Lymphocytes
HIV
Human immunodeficiency virus 1
HIV-1
Vaccinia virus
gag Genes
Human Immunodeficiency Virus Proteins
env Genes
Sequence Analysis
sequence analysis
blood
assays

ASJC Scopus subject areas

  • Immunology

Cite this

Betts, M. R., Krowka, J., Santamaria, C., Balsamo, K., Gao, F., Mulundu, G., ... Frelinger, J. A. (1997). Cross-clade human immunodeficiency virus (HIV)-specific cytotoxic T- lymphocyte responses in HIV-infected Zambians. Journal of Virology, 71(11), 8908-8911.

Cross-clade human immunodeficiency virus (HIV)-specific cytotoxic T- lymphocyte responses in HIV-infected Zambians. / Betts, M. R.; Krowka, J.; Santamaria, C.; Balsamo, K.; Gao, F.; Mulundu, G.; Luo, C.; N'Gandu, N.; Sheppard, H.; Hahn, B. H.; Allen, S.; Frelinger, Jeffrey A.

In: Journal of Virology, Vol. 71, No. 11, 1997, p. 8908-8911.

Research output: Contribution to journalArticle

Betts, MR, Krowka, J, Santamaria, C, Balsamo, K, Gao, F, Mulundu, G, Luo, C, N'Gandu, N, Sheppard, H, Hahn, BH, Allen, S & Frelinger, JA 1997, 'Cross-clade human immunodeficiency virus (HIV)-specific cytotoxic T- lymphocyte responses in HIV-infected Zambians', Journal of Virology, vol. 71, no. 11, pp. 8908-8911.
Betts MR, Krowka J, Santamaria C, Balsamo K, Gao F, Mulundu G et al. Cross-clade human immunodeficiency virus (HIV)-specific cytotoxic T- lymphocyte responses in HIV-infected Zambians. Journal of Virology. 1997;71(11):8908-8911.
Betts, M. R. ; Krowka, J. ; Santamaria, C. ; Balsamo, K. ; Gao, F. ; Mulundu, G. ; Luo, C. ; N'Gandu, N. ; Sheppard, H. ; Hahn, B. H. ; Allen, S. ; Frelinger, Jeffrey A. / Cross-clade human immunodeficiency virus (HIV)-specific cytotoxic T- lymphocyte responses in HIV-infected Zambians. In: Journal of Virology. 1997 ; Vol. 71, No. 11. pp. 8908-8911.
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