Cross-clade human immunodeficiency virus (HIV)-specific cytotoxic T- lymphocyte responses in HIV-infected Zambians

M. R. Betts, J. Krowka, C. Santamaria, K. Balsamo, F. Gao, G. Mulundu, C. Luo, N. N'Gandu, H. Sheppard, B. H. Hahn, S. Allen, J. A. Frelinger

Research output: Contribution to journalArticle

89 Scopus citations

Abstract

We have examined cross-clade HIV-specific cytotoxic T-lymphocyte (CTL) activity in peripheral blood of eight Zambian individuals infected with non- B-clade human immunodeficiency virus type 1 (HIV-1). Heteroduplex mobility assay and partial sequence analysis of env and gag genes strongly suggests that all the HIV-infected subjects were infected with clade C HIV-1. Six of eight C-clade HIV-infected individuals elicited CTL activity specific for recombinant vaccinia virus-infected autologous targets expressing HIV gag- pol-env derived from B-clade HIV-1 (IIIB). Recognition of individual recombinant HIV-1 B-clade vaccinia virus-infected targets expressing gag, pol, or env was variable among the patients tested, indicating that cross- clade CTL activity is not limited to a single HIV protein. These data demonstrate that HIV clade C-infected individuals can mount vigorous HIV clade B-reactive CTL responses.

Original languageEnglish (US)
Pages (from-to)8908-8911
Number of pages4
JournalJournal of virology
Volume71
Issue number11
StatePublished - Oct 28 1997
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Betts, M. R., Krowka, J., Santamaria, C., Balsamo, K., Gao, F., Mulundu, G., Luo, C., N'Gandu, N., Sheppard, H., Hahn, B. H., Allen, S., & Frelinger, J. A. (1997). Cross-clade human immunodeficiency virus (HIV)-specific cytotoxic T- lymphocyte responses in HIV-infected Zambians. Journal of virology, 71(11), 8908-8911.