Crumbs interacts with moesin and βHeavy-spectrin in the apical membrane skeleton of Drosophila

Emmanuelle Médina, Janice Williams, Elizabeth Klipfell, Daniela C Zarnescu, Graham Thomas, André Le Bivic

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

The apical transmembrane protein Crumbs is necessary for both cell polarization and the assembly of the zonula adherens (ZA) in Drosophila epithelia. The apical spectrin-based membrane skeleton (SBMS) is a protein network that is essential for epithelial morphogenesis and ZA integrity, and exhibits close colocalization with Crumbs and the ZA in fly epithelia. These observations suggest that Crumbs may stabilize the ZA by recruiting the SBMS to the junctional region. Consistent with this hypothesis, we report that Crumbs is necessary for the organization of the apical SBMS in embryos and Schneider 2 cells, whereas the localization of Crumbs is not affected in karst mutants that eliminate the apical SBMS. Our data indicate that it is specifically the 4.1 protein/ezrin/radixin/moesin (FERM) domain binding consensus, and in particular, an arginine at position 7 in the cytoplasmic tail of Crumbs that is essential to efficiently recruit both the apical SBMS and the FERM domain protein, DMoesin. Crumbs, Discs lost, βHeavy-spectrin, and DMoesin are all coimmunoprecipitated from embryos, confirming the existence of a multimolecular complex. We propose that Crumbs stabilizes the apical SBMS via DMoesin and actin, leading to reinforcement of the ZA and effectively coupling epithelial morphogenesis and cell polarity.

Original languageEnglish (US)
Pages (from-to)941-951
Number of pages11
JournalJournal of Cell Biology
Volume158
Issue number5
DOIs
StatePublished - Sep 2 2002
Externally publishedYes

Fingerprint

Spectrin
Skeleton
Adherens Junctions
Drosophila
Membranes
Morphogenesis
Embryonic Structures
Epithelium
Cell Polarity
Proteins
moesin
Diptera
Arginine
Tail
Actins
Epithelial Cells

Keywords

  • DMoesin
  • Drosophila
  • Epithelial polarity
  • Spectrin
  • Zonula adherens

ASJC Scopus subject areas

  • Cell Biology

Cite this

Crumbs interacts with moesin and βHeavy-spectrin in the apical membrane skeleton of Drosophila. / Médina, Emmanuelle; Williams, Janice; Klipfell, Elizabeth; Zarnescu, Daniela C; Thomas, Graham; Bivic, André Le.

In: Journal of Cell Biology, Vol. 158, No. 5, 02.09.2002, p. 941-951.

Research output: Contribution to journalArticle

Médina, Emmanuelle ; Williams, Janice ; Klipfell, Elizabeth ; Zarnescu, Daniela C ; Thomas, Graham ; Bivic, André Le. / Crumbs interacts with moesin and βHeavy-spectrin in the apical membrane skeleton of Drosophila. In: Journal of Cell Biology. 2002 ; Vol. 158, No. 5. pp. 941-951.
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AU - Bivic, André Le

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