Crustacean cardioactive peptide in the sphinx moth, Manduca sexta

H. K. Lehman, C. M. Murgiuc, T. A. Miller, T. D. Lee, John G Hildebrand

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

The isolation, identification, and actions of crustacean cardioactive peptide (CCAP) have been examined in the sphinx moth Manduca sexta. A sensitive and specific enzyme-linked immunosorbent assay (ELISA) was used to quantify CCAP-like immunoreactivity in the nervous system. The CCAP-like immunoreactivity from the abdominal CNS was then purified, and its sequence was ascertained by amino acid analysis, mass spectral analysis, and HPLC. These studies showed that the nervous system of M. sexta contains a peptide with the sequence Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys-NH2, identical to CCAP originally isolated and sequenced from the shore crab Carcinus maenas. The actions of CCAP on the isolated heart of M. sexta and the extensor-tibia muscle of Schistocerca americana were tested. Crustacean cardioactive peptide had excitatory actions on both preparations: a dose-dependent increase in the rate of contractions was observed on the heart, and an increase in the rate of the myogenic rhythm was observed in the leg muscle. Moreover, purified and synthetic CCAP had identical effects on the isolated heart. We conclude that CCAP occurs in M. sexta and exerts potent neurotransmitter or neurohormonal actions on a variety of muscles.

Original languageEnglish (US)
Pages (from-to)735-741
Number of pages7
JournalPeptides
Volume14
Issue number4
DOIs
StatePublished - 1993

Fingerprint

Manduca
Moths
Muscle
Neurology
Muscles
Nervous System
Brachyura
Immunosorbents
crustacean cardioactive peptide
Tibia
Spectrum analysis
Neurotransmitter Agents
Assays
Leg
Enzyme-Linked Immunosorbent Assay
High Pressure Liquid Chromatography
Amino Acids
Peptides

Keywords

  • Cardioactive peptide
  • CCAP
  • ELISA
  • HPLC
  • Manduca sexta
  • Neuropeptide

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Crustacean cardioactive peptide in the sphinx moth, Manduca sexta. / Lehman, H. K.; Murgiuc, C. M.; Miller, T. A.; Lee, T. D.; Hildebrand, John G.

In: Peptides, Vol. 14, No. 4, 1993, p. 735-741.

Research output: Contribution to journalArticle

Lehman, H. K. ; Murgiuc, C. M. ; Miller, T. A. ; Lee, T. D. ; Hildebrand, John G. / Crustacean cardioactive peptide in the sphinx moth, Manduca sexta. In: Peptides. 1993 ; Vol. 14, No. 4. pp. 735-741.
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