Cryopreservation of Moricandia arvensis (L.) DC (Brassicaceae) cell suspensions

Beneficial effects of pluronic F-68

Craig A Wissler, P. Anthony, C. M. O'Neill, R. J. Mathias, J. B. Power, K. C. Lowe, M. R. Davey

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cell suspension cultures of Moricandia arvensis, a photosynthetic C3-C4 intermediate within the Brassicaceae, were recovered from cryogenic storage. Post-thaw cell viability, determined by triphenyltetrazolium chloride reduction and cell re-growth, was influenced by composition of the cryoprotectant and, positively, by incorporation into the post-thaw medium of 0.01% (w/v) of the non-ionic surfactant, Pluronic F-68. The growth characteristics of re-established suspension cultures were similar to those of non-cryopreserved suspensions. Re-established suspensions also released viable protoplasts in yields comparable to the same non-cryopreserved cell suspensions.

Original languageEnglish (US)
Pages (from-to)201-208
Number of pages8
JournalCryo-Letters
Volume18
Issue number4
StatePublished - Jul 1997
Externally publishedYes

Fingerprint

Moricandia arvensis
Brassicaceae
Poloxamer
Cryopreservation
cryopreservation
cell suspension culture
Suspensions
nonionic surfactants
cryoprotectants
regrowth
protoplasts
cell viability
chlorides
Protoplasts
Growth
Surface-Active Agents
Cell Survival
Cell Culture Techniques
cells

Keywords

  • Brassicaceae
  • Cell suspensions
  • Cryopreservation
  • Moricandia arvensis
  • Pluronic F-68
  • Protoplast

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Physiology
  • Physiology (medical)

Cite this

Wissler, C. A., Anthony, P., O'Neill, C. M., Mathias, R. J., Power, J. B., Lowe, K. C., & Davey, M. R. (1997). Cryopreservation of Moricandia arvensis (L.) DC (Brassicaceae) cell suspensions: Beneficial effects of pluronic F-68. Cryo-Letters, 18(4), 201-208.

Cryopreservation of Moricandia arvensis (L.) DC (Brassicaceae) cell suspensions : Beneficial effects of pluronic F-68. / Wissler, Craig A; Anthony, P.; O'Neill, C. M.; Mathias, R. J.; Power, J. B.; Lowe, K. C.; Davey, M. R.

In: Cryo-Letters, Vol. 18, No. 4, 07.1997, p. 201-208.

Research output: Contribution to journalArticle

Wissler, CA, Anthony, P, O'Neill, CM, Mathias, RJ, Power, JB, Lowe, KC & Davey, MR 1997, 'Cryopreservation of Moricandia arvensis (L.) DC (Brassicaceae) cell suspensions: Beneficial effects of pluronic F-68', Cryo-Letters, vol. 18, no. 4, pp. 201-208.
Wissler, Craig A ; Anthony, P. ; O'Neill, C. M. ; Mathias, R. J. ; Power, J. B. ; Lowe, K. C. ; Davey, M. R. / Cryopreservation of Moricandia arvensis (L.) DC (Brassicaceae) cell suspensions : Beneficial effects of pluronic F-68. In: Cryo-Letters. 1997 ; Vol. 18, No. 4. pp. 201-208.
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