Cryptosporidium parvum metalloaminopeptidase inhibitors prevent in vitro excystation

Pablo C. Okhuysen, Cynthia L. Chappell, Charles Kettner, Charles R Sterling

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Cryptosporidium parvum arginine aminopeptidase (RAP) was studied during in vitro excystation. Specific RAP inhibitors were identified by using C. parvum extracts. Amastatin, a series of α-aminoboronic acids, and the chelating agents EDTA and 1.10-phenanthrolene, but not endoproteinase inhibitors, blocked enzymatic activity. RAP inhibitors found to be effective in soluble enzymatic assays were then studied for their effect on in vitro excystation. 1,10-Phenanthrolene, amastatin, and 11-boronorleucine (pinacol) inhibited excystation by 84, 57, and 61%, respectively, compared with solvent-treated control oocysts. Sporozoites remained viable within the oocyst an determined by propidium iodide and fluorescein diacetate dye uptake, suggesting that α-aminoboronic acids were not directly lethal to the parasite.

Original languageEnglish (US)
Pages (from-to)2781-2784
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume40
Issue number12
StatePublished - Dec 1996

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Cryptosporidium parvum
Oocysts
Sporozoites
Acids
Propidium
Enzyme Assays
Chelating Agents
Edetic Acid
Parasites
Coloring Agents
amastatin
In Vitro Techniques
aminopeptidase B
3',6'-diacetylfluorescein

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Cryptosporidium parvum metalloaminopeptidase inhibitors prevent in vitro excystation. / Okhuysen, Pablo C.; Chappell, Cynthia L.; Kettner, Charles; Sterling, Charles R.

In: Antimicrobial Agents and Chemotherapy, Vol. 40, No. 12, 12.1996, p. 2781-2784.

Research output: Contribution to journalArticle

Okhuysen, Pablo C. ; Chappell, Cynthia L. ; Kettner, Charles ; Sterling, Charles R. / Cryptosporidium parvum metalloaminopeptidase inhibitors prevent in vitro excystation. In: Antimicrobial Agents and Chemotherapy. 1996 ; Vol. 40, No. 12. pp. 2781-2784.
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