Cryptosporidium parvum mixed genotypes detected by PCR-restriction fragment length polymorphism analysis

C. Reed, G. D. Sturbaum, P. J. Hoover, Charles R Sterling

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Combinations of 10 Cryptosporidium parvum oocysts, with various ratios of genotype I to genotype II, were isolated and subjected to PCR-restriction fragment length polymorphism analysis. Amplification of both genotypes in these samples ranged from 31 to 74% and yielded no information about the genotype proportions. In addition, since both genotypes were not always detected, amplification of a single genotype is not conclusive evidence that the sample contains only a single genotype.

Original languageEnglish (US)
Pages (from-to)427-429
Number of pages3
JournalApplied and Environmental Microbiology
Volume68
Issue number1
DOIs
StatePublished - 2002

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Cryptosporidium parvum
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism
polymorphism
genotype
Genotype
Polymerase Chain Reaction
amplification
Oocysts
analysis
oocysts
sampling

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Cryptosporidium parvum mixed genotypes detected by PCR-restriction fragment length polymorphism analysis. / Reed, C.; Sturbaum, G. D.; Hoover, P. J.; Sterling, Charles R.

In: Applied and Environmental Microbiology, Vol. 68, No. 1, 2002, p. 427-429.

Research output: Contribution to journalArticle

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