Cryptosporidium parvum mixed genotypes detected by PCR-restriction fragment length polymorphism analysis

C. Reed, G. D. Sturbaum, P. J. Hoover, C. R. Sterling

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Combinations of 10 Cryptosporidium parvum oocysts, with various ratios of genotype I to genotype II, were isolated and subjected to PCR-restriction fragment length polymorphism analysis. Amplification of both genotypes in these samples ranged from 31 to 74% and yielded no information about the genotype proportions. In addition, since both genotypes were not always detected, amplification of a single genotype is not conclusive evidence that the sample contains only a single genotype.

Original languageEnglish (US)
Pages (from-to)427-429
Number of pages3
JournalApplied and environmental microbiology
Volume68
Issue number1
DOIs
StatePublished - Jan 15 2002

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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