Crystal structure and conformational flexibility of the unligated FK506-binding protein FKBP12.6

Hui Chen, Sourajit M. Mustafi, David M. LeMaster, Zhong Li, Annie Héroux, Hongmin Li, Griselda Hernández

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The primary known physiological function of FKBP12.6 involves its role in regulating the RyR2 isoform of ryanodine receptor Ca2+ channels in cardiac muscle, pancreatic β islets and the central nervous system. With only a single previously reported X-ray structure of FKBP12.6, bound to the immunosuppressant rapamycin, structural inferences for this protein have been drawn from the more extensive studies of the homologous FKBP12. X-ray structures at 1.70 and 1.90 Å resolution from P21 and P3121 crystal forms are reported for an unligated cysteine-free variant of FKBP12.6 which exhibit a notable diversity of conformations. In one monomer from the P3121 crystal form, the aromatic ring of Phe59 at the base of the active site is rotated perpendicular to its typical orientation, generating a steric conflict for the immunosuppressant-binding mode. The peptide unit linking Gly89 and Val90 at the tip of the protein-recognition '80s loop' is flipped in the P21 crystal form. Unlike the >30 reported FKBP12 structures, the backbone conformation of this loop closely follows that of the first FKBP domain of FKBP51. The NMR resonances for 21 backbone amides of FKBP12.6 are doubled, corresponding to a slow conformational transition centered near the tip of the 80s loop, as recently reported for 31 amides of FKBP12. The comparative absence of doubling for residues along the opposite face of the active-site pocket in FKBP12.6 may in part reflect attenuated structural coupling owing to increased conformational plasticity around the Phe59 ring.

Original languageEnglish (US)
Pages (from-to)636-646
Number of pages11
JournalActa Crystallographica Section D: Biological Crystallography
Volume70
Issue number3
DOIs
StatePublished - Mar 2014
Externally publishedYes

Keywords

  • FK506-binding proteins
  • FKBP12.6

ASJC Scopus subject areas

  • Structural Biology

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