Crystal structure of the lactose operon represser and its complexes with DNA and inducer

Mitchell Lewis, Geoffrey Chang, Nancy C Horton, Michele A. Kercher, Helen C. Pace, Maria A. Schumacher, Richard G. Brennan, Ponzy Lu

Research output: Contribution to journalArticle

613 Citations (Scopus)

Abstract

The lac operon of Escherichia coli is the paradigm for gene regulation. Its key component is the lac repressor, a product of the lacl gene. The three-dimensional structures of the intact lac repressor, the lac repressor bound to the gratuitous inducer isopropyl-β-D-1-thiogalactoside (IPTG) and the lac repressor complexed with a 21-base pair symmetric operator DNA have been determined. These three structures show the conformation of the molecule in both the induced and repressed states and provide a framework for understanding a wealth of biochemical and genetic information. The DNA sequence of the lac operon has three lac repressor recognition sites in a stretch of 500 base pairs. The crystallographic structure of the complex with DNA suggests that the tetrameric repressor functions synergistically with catabolite gene activator protein (CAP) and participates in the quaternary formation of repression loops in which one tetrameric repressor interacts simultaneously with two sites on the genomic DNA.

Original languageEnglish (US)
Pages (from-to)1247-1254
Number of pages8
JournalScience
Volume271
Issue number5253
StatePublished - Mar 1 1996
Externally publishedYes

Fingerprint

Lac Repressors
Lac Operon
DNA
Base Pairing
Cyclic AMP Receptor Protein
Thiogalactosides
Genes
Molecular Biology
Escherichia coli

ASJC Scopus subject areas

  • General

Cite this

Lewis, M., Chang, G., Horton, N. C., Kercher, M. A., Pace, H. C., Schumacher, M. A., ... Lu, P. (1996). Crystal structure of the lactose operon represser and its complexes with DNA and inducer. Science, 271(5253), 1247-1254.

Crystal structure of the lactose operon represser and its complexes with DNA and inducer. / Lewis, Mitchell; Chang, Geoffrey; Horton, Nancy C; Kercher, Michele A.; Pace, Helen C.; Schumacher, Maria A.; Brennan, Richard G.; Lu, Ponzy.

In: Science, Vol. 271, No. 5253, 01.03.1996, p. 1247-1254.

Research output: Contribution to journalArticle

Lewis, M, Chang, G, Horton, NC, Kercher, MA, Pace, HC, Schumacher, MA, Brennan, RG & Lu, P 1996, 'Crystal structure of the lactose operon represser and its complexes with DNA and inducer', Science, vol. 271, no. 5253, pp. 1247-1254.
Lewis M, Chang G, Horton NC, Kercher MA, Pace HC, Schumacher MA et al. Crystal structure of the lactose operon represser and its complexes with DNA and inducer. Science. 1996 Mar 1;271(5253):1247-1254.
Lewis, Mitchell ; Chang, Geoffrey ; Horton, Nancy C ; Kercher, Michele A. ; Pace, Helen C. ; Schumacher, Maria A. ; Brennan, Richard G. ; Lu, Ponzy. / Crystal structure of the lactose operon represser and its complexes with DNA and inducer. In: Science. 1996 ; Vol. 271, No. 5253. pp. 1247-1254.
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