Crystallization and preliminary diffraction analysis of the HincII restriction endonuclease-DNA complex

Nancy C. Horton, Lydia F. Dorner, Ira Schildkraut, John J. Perona

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Crystals of the 60 kDa dimeric HincII restriction enzyme bound to a 12 base-pair dyad-symmetric duplex DNA carrying the specific 5'-GTCGAC recognition site have been obtained. Crystals grew by hanging-drop vapor diffusion from solutions containing polyethylene glycol 4000 as precipitating agent. The rod-shaped crystals belong to space group I222 (or I212121), with unit-cell dimensions a = 66.9, b = 176.7, c = 256.0 Å. There are most likely to be two dimeric complexes in the asymmetric unit. A complete native data set has been collected from a high-energy synchrotron source to a resolution of 2.5 Å at 100 K, with an R(merge) of 4.8%.

Original languageEnglish (US)
Pages (from-to)1943-1945
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number11
DOIs
StatePublished - Nov 1 1999
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology

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