Crystallization of zinc finger proteins bound to DNA

Nancy C Horton, Chad K. Park

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

A method is presented for determining conditions for the cocrystallization of zinc finger proteins with DNA. The method describes steps beginning with protein expression, through purification, design of DNA for cocrystallization, and the conditions to screen for cocrystallization.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages457-477
Number of pages21
Volume649
DOIs
StatePublished - 2010

Publication series

NameMethods in Molecular Biology
Volume649
ISSN (Print)10643745

Fingerprint

Zinc Fingers
Crystallization
DNA
Proteins

Keywords

  • Crystallization
  • DNA
  • hanging drop vapor diffusion
  • X-ray crystal structure
  • zinc finger

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Horton, N. C., & Park, C. K. (2010). Crystallization of zinc finger proteins bound to DNA. In Methods in Molecular Biology (Vol. 649, pp. 457-477). (Methods in Molecular Biology; Vol. 649). https://doi.org/10.1007/978-1-60761-753-2_28

Crystallization of zinc finger proteins bound to DNA. / Horton, Nancy C; Park, Chad K.

Methods in Molecular Biology. Vol. 649 2010. p. 457-477 (Methods in Molecular Biology; Vol. 649).

Research output: Chapter in Book/Report/Conference proceedingChapter

Horton, NC & Park, CK 2010, Crystallization of zinc finger proteins bound to DNA. in Methods in Molecular Biology. vol. 649, Methods in Molecular Biology, vol. 649, pp. 457-477. https://doi.org/10.1007/978-1-60761-753-2_28
Horton NC, Park CK. Crystallization of zinc finger proteins bound to DNA. In Methods in Molecular Biology. Vol. 649. 2010. p. 457-477. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-60761-753-2_28
Horton, Nancy C ; Park, Chad K. / Crystallization of zinc finger proteins bound to DNA. Methods in Molecular Biology. Vol. 649 2010. pp. 457-477 (Methods in Molecular Biology).
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