Crystallization of zinc finger proteins bound to DNA

Nancy C. Horton, Chad K. Park

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

A method is presented for determining conditions for the cocrystallization of zinc finger proteins with DNA. The method describes steps beginning with protein expression, through purification, design of DNA for cocrystallization, and the conditions to screen for cocrystallization.

Original languageEnglish (US)
Title of host publicationEngineered Zinc Finger Proteins
Subtitle of host publicationMethods and Protocols
EditorsJoel Mackay, David Segal
Pages457-477
Number of pages21
DOIs
StatePublished - Dec 1 2010

Publication series

NameMethods in Molecular Biology
Volume649
ISSN (Print)1064-3745

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Keywords

  • Crystallization
  • DNA
  • X-ray crystal structure
  • hanging drop vapor diffusion
  • zinc finger

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Horton, N. C., & Park, C. K. (2010). Crystallization of zinc finger proteins bound to DNA. In J. Mackay, & D. Segal (Eds.), Engineered Zinc Finger Proteins: Methods and Protocols (pp. 457-477). (Methods in Molecular Biology; Vol. 649). https://doi.org/10.1007/978-1-60761-753-2_28