Cultivated single-stranded DNA phages that infect marine bacteroidetes prove difficult to detect with DNA-binding stains

Karin Holmfeldt, Duško Odić, Matthew B. Sullivan, Mathias Middelboe, Lasse Riemann

Research output: Contribution to journalArticle

39 Scopus citations


This is the first description of cultivated icosahedral single-stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA-binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.

Original languageEnglish (US)
Pages (from-to)892-894
Number of pages3
JournalApplied and environmental microbiology
Issue number3
StatePublished - Feb 1 2012


ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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