Culture medium composition affects the relative toxicities of chlorobenzenes in rat liver slices and the isolated perfused liver

Robyn L. Fisher, A Jay Gandolfi, I. Glenn Sipes, Klaus Brendel

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The effects of different media composition on the hepatotoxicity produced by monochlorobenzene (MCB), 1,2-dichlorobenzene (1,2-DCB), 1,3-dichlorobenzene (1,3-DCB) and 1,4-dichlorobenzene (1,4-DCB) were examined in two different in vitro systems. The toxicity of these chlorobenzenes was investigated in the perfused rat liver and liver slices using Krebs-Henseleit buffer. Significant differences between the chlorobenzenes were apparent in the perfused liver but not in the tissue slices. However, a dose and time related response of rat liver slices to the chlorobenzenes was observed. Partial amelioration of the chlorobenzene toxicity was observed when the Krebs-Henseleit buffer was supplemented with vitamins, amino acids, and/or bovine serum albumin. 1,2-DCB and 1,3-DCB toxicity was affected by amino acids and vitamins. The toxicity produced by 1,4-DCB was suppressed by amino acids, vitamins and 1% BSA. MCB hepatoxicity could only be suppressed by 1% BSA. This data suggests that tissue culture media composition plays a major role in the hepatotoxicity of the chlorobenzenes.

Original languageEnglish (US)
Pages (from-to)321-339
Number of pages19
JournalDrug and Chemical Toxicology
Volume16
Issue number4
DOIs
StatePublished - 1993

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Chlorobenzenes
Liver
Toxicity
Culture Media
Rats
Vitamins
Amino acids
Chemical analysis
Amino Acids
Tissue culture
Bovine Serum Albumin
Tissue
chlorobenzene
1,3-dichlorobenzene

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health
  • Chemical Health and Safety
  • Chemistry(all)

Cite this

Culture medium composition affects the relative toxicities of chlorobenzenes in rat liver slices and the isolated perfused liver. / Fisher, Robyn L.; Gandolfi, A Jay; Sipes, I. Glenn; Brendel, Klaus.

In: Drug and Chemical Toxicology, Vol. 16, No. 4, 1993, p. 321-339.

Research output: Contribution to journalArticle

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