Cutting edge: CXCR4 is a functional coreceptor for infection of human macrophages by CXCR4-dependent primary HIV-1 isolates

Alessia Verani, Elena Pesenti, Simona Polo, Eleonora Tresoldi, Gabriella Scarlatti, Paolo Lusso, Antonio G. Siccardi, Donata Vercelli

Research output: Contribution to journalArticlepeer-review

105 Scopus citations

Abstract

The identification of HIV-1 coreceptors has provided a molecular basis for the tropism of different HIV-1 strains. CXC chemokine receptor-4 (CXCR4) mediates the entry of both primary and T cell line-adapted (TCLA) syncytia- inducing strains. Although macrophages (Mφ) express CXCR4, this coreceptor is assumed to be nonfunctional for HIV-1 infection. We addressed this apparent paradox by infecting human monocyte-derived Mφ with primary and TCLA isolates that were rigorously characterized for coreceptor usage and by adding the natural CXCR4 ligand, stern cell differentiation factor-1, to specifically block CXCR4-mediated entry. Our results show that primary HIV-1 isolates that selectively use CXCR4 productively infected both normal and C- C chemokine receptor-5-null Mφ. By contrast, Mφ supported the entry of CXCR4-dependent TCLA strains with variable efficiency but were not productively infected. Thus, the tropism of HIV isolates results from complex virus/host cell interactions both at the entry and postentry levels.

Original languageEnglish (US)
Pages (from-to)2084-2088
Number of pages5
JournalJournal of Immunology
Volume161
Issue number5
StatePublished - Sep 1 1998
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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