Cutting edge: CXCR4 is a functional coreceptor for infection of human macrophages by CXCR4-dependent primary HIV-1 isolates

Alessia Verani, Elena Pesenti, Simona Polo, Eleonora Tresoldi, Gabriella Scarlatti, Paolo Lusso, Antonio G. Siccardi, Donata Vercelli

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

The identification of HIV-1 coreceptors has provided a molecular basis for the tropism of different HIV-1 strains. CXC chemokine receptor-4 (CXCR4) mediates the entry of both primary and T cell line-adapted (TCLA) syncytia- inducing strains. Although macrophages (Mφ) express CXCR4, this coreceptor is assumed to be nonfunctional for HIV-1 infection. We addressed this apparent paradox by infecting human monocyte-derived Mφ with primary and TCLA isolates that were rigorously characterized for coreceptor usage and by adding the natural CXCR4 ligand, stern cell differentiation factor-1, to specifically block CXCR4-mediated entry. Our results show that primary HIV-1 isolates that selectively use CXCR4 productively infected both normal and C- C chemokine receptor-5-null Mφ. By contrast, Mφ supported the entry of CXCR4-dependent TCLA strains with variable efficiency but were not productively infected. Thus, the tropism of HIV isolates results from complex virus/host cell interactions both at the entry and postentry levels.

Original languageEnglish (US)
Pages (from-to)2084-2088
Number of pages5
JournalJournal of Immunology
Volume161
Issue number5
StatePublished - Sep 1 1998
Externally publishedYes

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CXCR4 Receptors
HIV-1
Macrophages
Infection
Tropism
T-Lymphocytes
Cell Line
CC Chemokines
Chemokine Receptors
Giant Cells
Cell Communication
HIV Infections
Monocytes
Cell Differentiation
HIV
Ligands
Viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Cutting edge : CXCR4 is a functional coreceptor for infection of human macrophages by CXCR4-dependent primary HIV-1 isolates. / Verani, Alessia; Pesenti, Elena; Polo, Simona; Tresoldi, Eleonora; Scarlatti, Gabriella; Lusso, Paolo; Siccardi, Antonio G.; Vercelli, Donata.

In: Journal of Immunology, Vol. 161, No. 5, 01.09.1998, p. 2084-2088.

Research output: Contribution to journalArticle

Verani, A, Pesenti, E, Polo, S, Tresoldi, E, Scarlatti, G, Lusso, P, Siccardi, AG & Vercelli, D 1998, 'Cutting edge: CXCR4 is a functional coreceptor for infection of human macrophages by CXCR4-dependent primary HIV-1 isolates', Journal of Immunology, vol. 161, no. 5, pp. 2084-2088.
Verani A, Pesenti E, Polo S, Tresoldi E, Scarlatti G, Lusso P et al. Cutting edge: CXCR4 is a functional coreceptor for infection of human macrophages by CXCR4-dependent primary HIV-1 isolates. Journal of Immunology. 1998 Sep 1;161(5):2084-2088.
Verani, Alessia ; Pesenti, Elena ; Polo, Simona ; Tresoldi, Eleonora ; Scarlatti, Gabriella ; Lusso, Paolo ; Siccardi, Antonio G. ; Vercelli, Donata. / Cutting edge : CXCR4 is a functional coreceptor for infection of human macrophages by CXCR4-dependent primary HIV-1 isolates. In: Journal of Immunology. 1998 ; Vol. 161, No. 5. pp. 2084-2088.
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