Cutting edge: Single-chain trimers of MHC class I molecules form stable structures that potently stimulate antigen-specific T cells and B cells

Yik Y.L. Yu, Nikolai Netuschil, Lonnie Lybarger, Janet M. Connolly, Ted H. Hansen

Research output: Contribution to journalArticlepeer-review

83 Scopus citations

Abstract

We report in this work the expression and characterization of class I molecules expressed as single-chain trimers consisting of an antigenic peptide-spacer-β2-microglobulin-spacer H chain. Our results indicate that these single-chain constructs assemble efficiently, maintain their covalent structure, and are unusually stable at the cell surface. Consequently, these constructs are at least 1000-fold less accessible to exogenous peptide than class I molecules loaded with endogenous peptides, and they are potent simulators of peptide-specific CTL and Abs. Our combined findings suggest that single-chain trimers may have applications as DNA vaccines against virus infection or tumors.

Original languageEnglish (US)
Pages (from-to)3145-3149
Number of pages5
JournalJournal of Immunology
Volume168
Issue number7
DOIs
StatePublished - Apr 1 2002

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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