Cyclic adenosine 3': 5' monophosphate phosphodiesterase. Distinct forms in human lymphocytes and monocytes

W. J. Thompson, C. P. Ross, W. J. Pledger, S. J. Strada, R. L. Banner, Evan M Hersh

Research output: Contribution to journalArticle

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Abstract

Adenosine 3',5' monophosphate (cyclic AMP) phosphodiesterase activity of normal human peripheral blood leukocyte suspensions containing 90% lymphocytes and 10% monocytes showed anomalous kinetic behavior indicative of multiple enzyme forms. Kinetic analyses of purified lymphocyte (99%) or monocyte preparations (95%) indicated that only one type of phosphodiesterase was present in each cell type. None of the preparations contained any detectable guanosine 3',5' monophosphate (cyclic GMP) hydrolytic activity. The lymphocyte enzyme had an apparent K(m) 0 0.4 μM for cyclic AMP and V(max) 0 0.5 picomoles/min/10 6 cells. These kinetic parameters were confirmed by several cell purification techniques used alone and sequentially. Sedimentation velocity analyses indicated that the higher K(m) monocyte enzyme had a molecular weight near 45,000 and that the lower K(m) lymphocyte enzyme most likely had a molecular weight near 98,000. A variety of procedures led to a loss of the higher molecular weight, high affinity enzyme leaving only the enzyme of 45,000 daltons with a much lower substrate affinity. A long term, stable human lymphoblastoid cell line had cyclic AMP phosphodiesterase activity that was similar to the lymphocyte enzyme by both physical and kinetic criteria. Lymphocyte cyclic AMP phosphodiesterase appears to be a soluble enzyme whose pH and temperature optima and cationic requirements are similar to those of other mammalian phosphodiesterases. The distinct cyclic AMP phosphodiesterase forms of these cells may possibly represent the basic, active subunit of mammalian cyclic nucleotide phosphodiesterases. The authors hypothesize that the extremely high affinity cyclic AMP phosphodiesterase of normal lymphocytes plays an important role in the regulation of normal function in these cells, and also in the rapid proliferative responses characteristic of the stimulated lymphocyte.

Original languageEnglish (US)
Pages (from-to)4922-4929
Number of pages8
JournalJournal of Biological Chemistry
Volume251
Issue number16
StatePublished - 1976
Externally publishedYes

Fingerprint

Type 5 Cyclic Nucleotide Phosphodiesterases
Lymphocytes
Phosphoric Diester Hydrolases
Adenosine
Monocytes
Cyclic AMP
Enzymes
Molecular Weight
Molecular weight
Kinetics
3',5'-Cyclic-AMP Phosphodiesterases
Guanosine Monophosphate
Cyclic Nucleotides
Cyclic GMP
Sedimentation
Kinetic parameters
Human Activities
Purification
Suspensions
Leukocytes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Thompson, W. J., Ross, C. P., Pledger, W. J., Strada, S. J., Banner, R. L., & Hersh, E. M. (1976). Cyclic adenosine 3': 5' monophosphate phosphodiesterase. Distinct forms in human lymphocytes and monocytes. Journal of Biological Chemistry, 251(16), 4922-4929.

Cyclic adenosine 3' : 5' monophosphate phosphodiesterase. Distinct forms in human lymphocytes and monocytes. / Thompson, W. J.; Ross, C. P.; Pledger, W. J.; Strada, S. J.; Banner, R. L.; Hersh, Evan M.

In: Journal of Biological Chemistry, Vol. 251, No. 16, 1976, p. 4922-4929.

Research output: Contribution to journalArticle

Thompson, WJ, Ross, CP, Pledger, WJ, Strada, SJ, Banner, RL & Hersh, EM 1976, 'Cyclic adenosine 3': 5' monophosphate phosphodiesterase. Distinct forms in human lymphocytes and monocytes', Journal of Biological Chemistry, vol. 251, no. 16, pp. 4922-4929.
Thompson, W. J. ; Ross, C. P. ; Pledger, W. J. ; Strada, S. J. ; Banner, R. L. ; Hersh, Evan M. / Cyclic adenosine 3' : 5' monophosphate phosphodiesterase. Distinct forms in human lymphocytes and monocytes. In: Journal of Biological Chemistry. 1976 ; Vol. 251, No. 16. pp. 4922-4929.
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AB - Adenosine 3',5' monophosphate (cyclic AMP) phosphodiesterase activity of normal human peripheral blood leukocyte suspensions containing 90% lymphocytes and 10% monocytes showed anomalous kinetic behavior indicative of multiple enzyme forms. Kinetic analyses of purified lymphocyte (99%) or monocyte preparations (95%) indicated that only one type of phosphodiesterase was present in each cell type. None of the preparations contained any detectable guanosine 3',5' monophosphate (cyclic GMP) hydrolytic activity. The lymphocyte enzyme had an apparent K(m) 0 0.4 μM for cyclic AMP and V(max) 0 0.5 picomoles/min/10 6 cells. These kinetic parameters were confirmed by several cell purification techniques used alone and sequentially. Sedimentation velocity analyses indicated that the higher K(m) monocyte enzyme had a molecular weight near 45,000 and that the lower K(m) lymphocyte enzyme most likely had a molecular weight near 98,000. A variety of procedures led to a loss of the higher molecular weight, high affinity enzyme leaving only the enzyme of 45,000 daltons with a much lower substrate affinity. A long term, stable human lymphoblastoid cell line had cyclic AMP phosphodiesterase activity that was similar to the lymphocyte enzyme by both physical and kinetic criteria. Lymphocyte cyclic AMP phosphodiesterase appears to be a soluble enzyme whose pH and temperature optima and cationic requirements are similar to those of other mammalian phosphodiesterases. The distinct cyclic AMP phosphodiesterase forms of these cells may possibly represent the basic, active subunit of mammalian cyclic nucleotide phosphodiesterases. The authors hypothesize that the extremely high affinity cyclic AMP phosphodiesterase of normal lymphocytes plays an important role in the regulation of normal function in these cells, and also in the rapid proliferative responses characteristic of the stimulated lymphocyte.

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