Cyclic AMP inhibits c-Ha-ras protooncogene expression, and DNA synthesis in rat aortic smooth muscle cells

D. N. Sadhu, K. S. Ramos

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Fetal bovine serum (10%) markedly increased ras transcript levels in growth-arrested (Go-synchronized) smooth muscle cells by 8 h. This elevation was maintained for up to 18 h and returned, to pre-stimulation levels within 24 h. Challenge of quiescent cells with serum in the presence of dibetyryl cyclic AMP (1-100 μM), a growth inhibitor for smooth muscle cells, attenuated serum-induced elevation of c-Ha-ras in a dose dependent fashion and preventod progression of the cells into S phase. These results demonstrate that expression of c-Ha-ras in rat aortic smooth muscle cells is cell-cycle dependent and that cAMP prevents the induction of this protooncogene by serum.

Original languageEnglish (US)
Pages (from-to)567-570
Number of pages4
JournalExperientia
Volume49
Issue number6-7
DOIs
StatePublished - Jul 1993

Keywords

  • aortic smooth muscle cells
  • c-Ha-ras
  • cell cycle
  • cyclic AMP
  • proliferation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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