Cyclooxygenase 2 expression in cervical intraepithelial neoplasia and vulvar cancer

Amy L Mitchell, J. Michael Newton, Kathleen Brite, Janine G Einspahr, Malathi Ellis, John Davis, Thomas Nuno, David S Alberts, Francisco A Garcia

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

OBJECTIVE. The purpose of this study is to describe the expression of cyclooxygenase (COX) 2 cervical intraepithelial neoplasia (CIN) and vulvar cancer specimens. MATERIALS AND METHODS. Archived tissues from 21 cases including 6 cases negative for CIN (no CIN), 71 low-grade (CIN 1) diseases, 8 high-grade (CIN 2, 3) diseases, and 14 vulvar cancer cases were examined. Immunohistochemistry was evaluated in COX-2 expression in tissue using an isoform-specific COX-2 polyclonal antibody. Specimens were assigned an immunohistochemical score for intensity of staining and the percent of cells stained. The slides were scored by 2 independent pathologists and compared across histological categories. RESULTS. A greater proportion of cells were stained in specimens with high-grade CIN (p = 0.01). Staining intensity was not statistically different among the 3 groups. Higher scores were found for vulvar cancer as compared with normal vulva (p = 0.01). CONCLUSIONS. The increase in COX-2 in cervical cancer precursors may provide a potential target for prevention studies.

Original languageEnglish (US)
Pages (from-to)80-85
Number of pages6
JournalJournal of Lower Genital Tract Disease
Volume11
Issue number2
DOIs
StatePublished - Apr 2007

Fingerprint

Vulvar Neoplasms
Cervical Intraepithelial Neoplasia
Cyclooxygenase 2
Staining and Labeling
Vulva
Uterine Cervical Neoplasms
Protein Isoforms
Immunohistochemistry
Antibodies

Keywords

  • Cervical intraepithelial neoplasia
  • COX-2
  • Molecular target
  • Prevention
  • Vulvar cancer

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Cyclooxygenase 2 expression in cervical intraepithelial neoplasia and vulvar cancer. / Mitchell, Amy L; Newton, J. Michael; Brite, Kathleen; Einspahr, Janine G; Ellis, Malathi; Davis, John; Nuno, Thomas; Alberts, David S; Garcia, Francisco A.

In: Journal of Lower Genital Tract Disease, Vol. 11, No. 2, 04.2007, p. 80-85.

Research output: Contribution to journalArticle

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abstract = "OBJECTIVE. The purpose of this study is to describe the expression of cyclooxygenase (COX) 2 cervical intraepithelial neoplasia (CIN) and vulvar cancer specimens. MATERIALS AND METHODS. Archived tissues from 21 cases including 6 cases negative for CIN (no CIN), 71 low-grade (CIN 1) diseases, 8 high-grade (CIN 2, 3) diseases, and 14 vulvar cancer cases were examined. Immunohistochemistry was evaluated in COX-2 expression in tissue using an isoform-specific COX-2 polyclonal antibody. Specimens were assigned an immunohistochemical score for intensity of staining and the percent of cells stained. The slides were scored by 2 independent pathologists and compared across histological categories. RESULTS. A greater proportion of cells were stained in specimens with high-grade CIN (p = 0.01). Staining intensity was not statistically different among the 3 groups. Higher scores were found for vulvar cancer as compared with normal vulva (p = 0.01). CONCLUSIONS. The increase in COX-2 in cervical cancer precursors may provide a potential target for prevention studies.",
keywords = "Cervical intraepithelial neoplasia, COX-2, Molecular target, Prevention, Vulvar cancer",
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T1 - Cyclooxygenase 2 expression in cervical intraepithelial neoplasia and vulvar cancer

AU - Mitchell, Amy L

AU - Newton, J. Michael

AU - Brite, Kathleen

AU - Einspahr, Janine G

AU - Ellis, Malathi

AU - Davis, John

AU - Nuno, Thomas

AU - Alberts, David S

AU - Garcia, Francisco A

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N2 - OBJECTIVE. The purpose of this study is to describe the expression of cyclooxygenase (COX) 2 cervical intraepithelial neoplasia (CIN) and vulvar cancer specimens. MATERIALS AND METHODS. Archived tissues from 21 cases including 6 cases negative for CIN (no CIN), 71 low-grade (CIN 1) diseases, 8 high-grade (CIN 2, 3) diseases, and 14 vulvar cancer cases were examined. Immunohistochemistry was evaluated in COX-2 expression in tissue using an isoform-specific COX-2 polyclonal antibody. Specimens were assigned an immunohistochemical score for intensity of staining and the percent of cells stained. The slides were scored by 2 independent pathologists and compared across histological categories. RESULTS. A greater proportion of cells were stained in specimens with high-grade CIN (p = 0.01). Staining intensity was not statistically different among the 3 groups. Higher scores were found for vulvar cancer as compared with normal vulva (p = 0.01). CONCLUSIONS. The increase in COX-2 in cervical cancer precursors may provide a potential target for prevention studies.

AB - OBJECTIVE. The purpose of this study is to describe the expression of cyclooxygenase (COX) 2 cervical intraepithelial neoplasia (CIN) and vulvar cancer specimens. MATERIALS AND METHODS. Archived tissues from 21 cases including 6 cases negative for CIN (no CIN), 71 low-grade (CIN 1) diseases, 8 high-grade (CIN 2, 3) diseases, and 14 vulvar cancer cases were examined. Immunohistochemistry was evaluated in COX-2 expression in tissue using an isoform-specific COX-2 polyclonal antibody. Specimens were assigned an immunohistochemical score for intensity of staining and the percent of cells stained. The slides were scored by 2 independent pathologists and compared across histological categories. RESULTS. A greater proportion of cells were stained in specimens with high-grade CIN (p = 0.01). Staining intensity was not statistically different among the 3 groups. Higher scores were found for vulvar cancer as compared with normal vulva (p = 0.01). CONCLUSIONS. The increase in COX-2 in cervical cancer precursors may provide a potential target for prevention studies.

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KW - Molecular target

KW - Prevention

KW - Vulvar cancer

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