Cyclosporine inhibits prolactin induction of ornithine decarboxylase in rat tissues

Diane Haddock Russell, Douglas F Larson, Stephan B. Cardon, Jack G. Copeland

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Cyclosporine (CyA), formerly cyclosporin A, significantly inhibited the ability of prolactin (PRL) to elevate ornithine decarboxylase (ODC) activity in a variety of rat tissues. Administration of PRL to hypophysectomized rats also resulted in an induction of ODC activity which was inhibited markedly in all tissues studied in the presence of CyA. Transglutaminase (TGase) activity was not affected in any significant manner by PRL or CyA in most tissues studied. However, it was elevated in the adrenal by 10-8 M PRL. Bromocryptine, which selectively antagonizes pituitary PRL release, decreased the kidney ODC basal levels to 30% of vehicle control and serum PRL level to 4.3 ± 1.4 compared to 28 ± 10 in controls, suggestive of PRL maintenance of steady-state ODC activity in the kidney. CyA administration did not affect the action of glucagon, a known cyclic AMP-mediated hormone, or 8-bromo-cyclic AMP on kidney ODC activity. The elevation of rat kidney ODC activity by dexamethasone and triiodothyronine (T3), compounds which elevated serum prolactin levels in all cases, was also blocked by administration of CyA. Epidermal growth factor (EGF), which did not induce rat kidney ODC activity by itself, was capable of producing a small increment in ODC activity in the presence of CyA. The marked effect of CyA to selectively block ODC induction by PRL may be due to the ability of CyA to interact with receptor-required phospholipids in membranes and thus to antagonize hormone-receptor interaction.

Original languageEnglish (US)
Pages (from-to)159-166
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume35
Issue number2-3
DOIs
StatePublished - 1984

Fingerprint

Ornithine Decarboxylase
Prolactin
Cyclosporine
Rats
Tissue
Kidney
Hormones
8-Bromo Cyclic Adenosine Monophosphate
Transglutaminases
Bromocriptine
Triiodothyronine
Glucagon
Serum
Epidermal Growth Factor
Cyclic AMP
Dexamethasone
Phospholipids
Maintenance
Membranes

Keywords

  • bromocryptine
  • cyclic AMP-mediated hormones
  • cyclosporine
  • growth factors
  • hypophysectomy
  • RNA content
  • steroid hormones
  • transglutaminase

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Cyclosporine inhibits prolactin induction of ornithine decarboxylase in rat tissues. / Haddock Russell, Diane; Larson, Douglas F; Cardon, Stephan B.; Copeland, Jack G.

In: Molecular and Cellular Endocrinology, Vol. 35, No. 2-3, 1984, p. 159-166.

Research output: Contribution to journalArticle

Haddock Russell, Diane ; Larson, Douglas F ; Cardon, Stephan B. ; Copeland, Jack G. / Cyclosporine inhibits prolactin induction of ornithine decarboxylase in rat tissues. In: Molecular and Cellular Endocrinology. 1984 ; Vol. 35, No. 2-3. pp. 159-166.
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