CYP287A1 is a carotenoid 2-β-hydroxylase required for deinoxanthin biosynthesis in Deinococcus radiodurans R1

Zhengfu Zhou, Wei Zhang, Shiyou Su, Ming Chen, Wei Lu, Min Lin, Istvan Molnar, Yuquan Xu

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The carotenoid deinoxanthin is a crucial resistance factor against various stresses in the radiation-resistant bacterium Deinococcus radiodurans. Disruption of the gene dr2473 encoding the cytochrome P450 CYP287A1 led to the accumulation of 2-deoxydeinoxanthin in D. radiodurans, demonstrating that CYP287A1 is a novel β-carotene 2-hydroxylase. The dr2473 knockout mutant was shown to be more sensitive to UV radiation and oxidative stress than the wild-type strain D. radiodurans R1, indicating that the C2 alcohol of deinoxanthin is important for antioxidant activity.

Original languageEnglish (US)
Pages (from-to)10539-10546
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume99
Issue number24
DOIs
StatePublished - Dec 1 2015

Keywords

  • 2-β-hydroxylase
  • CYP287A1
  • Cytochrome P450
  • Deinococcus radiodurans
  • Deinoxanthin

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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