CYP90C1 and CYP90D1 are involved in different steps in the brassinosteroid biosynthesis pathway in Arabidopsis thaliana

Gyung Tae Kim, Shozo Fujioka, Toshiaki Kozuka, Frans Tax, Suguru Takatsuto, Shigeo Yoshida, Hirokazu Tsukaya

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Brassinosteroids (BRs) are plant hormones that are essential for a wide range of developmental processes in plants. Many of the genes responsible for the early reactions in the biosynthesis of BRs have recently been identified. However, several genes for enzymes that catalyze late steps in the biosynthesis pathways of BRs remain to be identified, and only a few genes responsible for the reactions that produce bioactive BRs have been identified. We found that the ROTUNDIFOLIA3 (ROT3) gene, encoding the enzyme CYP90C1, which was specifically involved in the regulation of leaf length in Arabidopsis thaliana, was required for the late steps in the BR biosynthesis pathway. ROT3 appears to be required for the conversion of typhasterol to castasterone, an activation step in the BR pathway. We also analyzed the gene most closely related to ROT3, CYP90D1, and found that double mutants for ROT3 and CYP90D1 had a severe dwarf phenotype, whereas cyp90d1 single knockout mutants did not. BR profiling in these mutants revealed that CYP90D1 was also involved in BR biosynthesis pathways. ROT3 and CYP90D1 were expressed differentially in leaves of A. thaliana, and the mutants for these two genes differed in their defects in elongation of hypocotyls under light conditions. The expression of CYP90D1 was strongly induced in leaf petioles in the dark. The results of the present study provide evidence that the two cytochrome P450s, CYP90C1 and CYP90D1, play distinct roles in organ-specific environmental regulation of the biosynthesis of BRs.

Original languageEnglish (US)
Pages (from-to)710-721
Number of pages12
JournalPlant Journal
Volume41
Issue number5
DOIs
StatePublished - Mar 2005

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Brassinosteroids
brassinosteroids
Arabidopsis
Arabidopsis thaliana
biosynthesis
Genes
genes
mutants
Hypocotyl
leaves
Plant Growth Regulators
knockout mutants
environmental law
Enzymes
Cytochromes
petioles
cytochromes
enzymes
plant hormones
hypocotyls

Keywords

  • Arabidopsis thaliana
  • Brassinosteroid
  • CYP90C1
  • CYP90D1
  • Leaf development
  • ROTUNDIFOLIA3

ASJC Scopus subject areas

  • Plant Science

Cite this

CYP90C1 and CYP90D1 are involved in different steps in the brassinosteroid biosynthesis pathway in Arabidopsis thaliana. / Kim, Gyung Tae; Fujioka, Shozo; Kozuka, Toshiaki; Tax, Frans; Takatsuto, Suguru; Yoshida, Shigeo; Tsukaya, Hirokazu.

In: Plant Journal, Vol. 41, No. 5, 03.2005, p. 710-721.

Research output: Contribution to journalArticle

Kim, Gyung Tae ; Fujioka, Shozo ; Kozuka, Toshiaki ; Tax, Frans ; Takatsuto, Suguru ; Yoshida, Shigeo ; Tsukaya, Hirokazu. / CYP90C1 and CYP90D1 are involved in different steps in the brassinosteroid biosynthesis pathway in Arabidopsis thaliana. In: Plant Journal. 2005 ; Vol. 41, No. 5. pp. 710-721.
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