Cytochrome P450 and actin genes expressed in Helicoverpa zea and Helicoverpa armigera: Paralogy/orthology identification, gene conversion and evolution

Xianchun Li, May R. Berenbaum, Mary A. Schuler

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Molecular phylogenetic analysis was conducted using conserved cytoplasmic actin and diversified cytochrome P450 (P450) sequences isolated from Helicoverpa zea and Helicoverpa armigera, two species thought to be closely related based on allozyme analyses. These sequences were compared in turn with published sequences from other insects to gain insight into how different gene families evolve. In Bombyx mori and these Helicoverpa species, cytoplasmic actin genes are present as a pair of tandemly duplicated paralogs with coding sequence identities as high as 95.5% (B. mori), 98.9% (H. zea) and 98.5% (H. armigera) due to recent 5′-polar gene conversions. Phylogeny and interspecies comparisons assign the six actin genes into two orthologous groups: HaA3a/HzA3a/BmA3 and HaA3b/HzA3b/BmA4, which exhibit more similarities between H. zea and H. armigera than between Helicoverpa species and B. mori. Like the actin genes in H. zea, four CYP6B genes exist as two pairs of duplicated paralogs with recent 5′-polar gene conversions. Interspecific comparisons and phylogeny analysis identified three groups of orthologous CYP6B genes: H. zea CYP6B8 or CYP6B28/H. armigera CYP6B7, H. zea CYP6B27/H. armigera CYP6B6, and H. zea CYP6B9/H. armigera CYP6B2/Heliothis virescens CYP6B10. The low degree of divergence in the first two of these groups is comparable to allelic variation within a single species. These orthologous relationships and the high degrees of similarity in both actin and P450 genes strongly indicate that these Helicoverpa species are extremely closely related.

Original languageEnglish (US)
Pages (from-to)311-320
Number of pages10
JournalInsect Biochemistry and Molecular Biology
Volume32
Issue number3
DOIs
StatePublished - Mar 1 2002
Externally publishedYes

Fingerprint

Gene Conversion
gene conversion
Helicoverpa zea
Helicoverpa armigera
cytochrome P-450
Cytochrome P-450 Enzyme System
Zea mays
actin
Actins
Genes
Helicoverpa
Bombyx
Bombyx mori
genes
Phylogeny
phylogeny
Heliothis virescens
allozymes
Isoenzymes
Insects

Keywords

  • Actin
  • Cytochrome P450
  • Evolution
  • Gene conversion
  • Heliothinae

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry

Cite this

Cytochrome P450 and actin genes expressed in Helicoverpa zea and Helicoverpa armigera : Paralogy/orthology identification, gene conversion and evolution. / Li, Xianchun; Berenbaum, May R.; Schuler, Mary A.

In: Insect Biochemistry and Molecular Biology, Vol. 32, No. 3, 01.03.2002, p. 311-320.

Research output: Contribution to journalArticle

@article{42ebb5bd7b984067b34b5aade68f67d3,
title = "Cytochrome P450 and actin genes expressed in Helicoverpa zea and Helicoverpa armigera: Paralogy/orthology identification, gene conversion and evolution",
abstract = "Molecular phylogenetic analysis was conducted using conserved cytoplasmic actin and diversified cytochrome P450 (P450) sequences isolated from Helicoverpa zea and Helicoverpa armigera, two species thought to be closely related based on allozyme analyses. These sequences were compared in turn with published sequences from other insects to gain insight into how different gene families evolve. In Bombyx mori and these Helicoverpa species, cytoplasmic actin genes are present as a pair of tandemly duplicated paralogs with coding sequence identities as high as 95.5{\%} (B. mori), 98.9{\%} (H. zea) and 98.5{\%} (H. armigera) due to recent 5′-polar gene conversions. Phylogeny and interspecies comparisons assign the six actin genes into two orthologous groups: HaA3a/HzA3a/BmA3 and HaA3b/HzA3b/BmA4, which exhibit more similarities between H. zea and H. armigera than between Helicoverpa species and B. mori. Like the actin genes in H. zea, four CYP6B genes exist as two pairs of duplicated paralogs with recent 5′-polar gene conversions. Interspecific comparisons and phylogeny analysis identified three groups of orthologous CYP6B genes: H. zea CYP6B8 or CYP6B28/H. armigera CYP6B7, H. zea CYP6B27/H. armigera CYP6B6, and H. zea CYP6B9/H. armigera CYP6B2/Heliothis virescens CYP6B10. The low degree of divergence in the first two of these groups is comparable to allelic variation within a single species. These orthologous relationships and the high degrees of similarity in both actin and P450 genes strongly indicate that these Helicoverpa species are extremely closely related.",
keywords = "Actin, Cytochrome P450, Evolution, Gene conversion, Heliothinae",
author = "Xianchun Li and Berenbaum, {May R.} and Schuler, {Mary A.}",
year = "2002",
month = "3",
day = "1",
doi = "10.1016/S0965-1748(01)00092-3",
language = "English (US)",
volume = "32",
pages = "311--320",
journal = "Insect Biochemistry and Molecular Biology",
issn = "0965-1748",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Cytochrome P450 and actin genes expressed in Helicoverpa zea and Helicoverpa armigera

T2 - Paralogy/orthology identification, gene conversion and evolution

AU - Li, Xianchun

AU - Berenbaum, May R.

AU - Schuler, Mary A.

PY - 2002/3/1

Y1 - 2002/3/1

N2 - Molecular phylogenetic analysis was conducted using conserved cytoplasmic actin and diversified cytochrome P450 (P450) sequences isolated from Helicoverpa zea and Helicoverpa armigera, two species thought to be closely related based on allozyme analyses. These sequences were compared in turn with published sequences from other insects to gain insight into how different gene families evolve. In Bombyx mori and these Helicoverpa species, cytoplasmic actin genes are present as a pair of tandemly duplicated paralogs with coding sequence identities as high as 95.5% (B. mori), 98.9% (H. zea) and 98.5% (H. armigera) due to recent 5′-polar gene conversions. Phylogeny and interspecies comparisons assign the six actin genes into two orthologous groups: HaA3a/HzA3a/BmA3 and HaA3b/HzA3b/BmA4, which exhibit more similarities between H. zea and H. armigera than between Helicoverpa species and B. mori. Like the actin genes in H. zea, four CYP6B genes exist as two pairs of duplicated paralogs with recent 5′-polar gene conversions. Interspecific comparisons and phylogeny analysis identified three groups of orthologous CYP6B genes: H. zea CYP6B8 or CYP6B28/H. armigera CYP6B7, H. zea CYP6B27/H. armigera CYP6B6, and H. zea CYP6B9/H. armigera CYP6B2/Heliothis virescens CYP6B10. The low degree of divergence in the first two of these groups is comparable to allelic variation within a single species. These orthologous relationships and the high degrees of similarity in both actin and P450 genes strongly indicate that these Helicoverpa species are extremely closely related.

AB - Molecular phylogenetic analysis was conducted using conserved cytoplasmic actin and diversified cytochrome P450 (P450) sequences isolated from Helicoverpa zea and Helicoverpa armigera, two species thought to be closely related based on allozyme analyses. These sequences were compared in turn with published sequences from other insects to gain insight into how different gene families evolve. In Bombyx mori and these Helicoverpa species, cytoplasmic actin genes are present as a pair of tandemly duplicated paralogs with coding sequence identities as high as 95.5% (B. mori), 98.9% (H. zea) and 98.5% (H. armigera) due to recent 5′-polar gene conversions. Phylogeny and interspecies comparisons assign the six actin genes into two orthologous groups: HaA3a/HzA3a/BmA3 and HaA3b/HzA3b/BmA4, which exhibit more similarities between H. zea and H. armigera than between Helicoverpa species and B. mori. Like the actin genes in H. zea, four CYP6B genes exist as two pairs of duplicated paralogs with recent 5′-polar gene conversions. Interspecific comparisons and phylogeny analysis identified three groups of orthologous CYP6B genes: H. zea CYP6B8 or CYP6B28/H. armigera CYP6B7, H. zea CYP6B27/H. armigera CYP6B6, and H. zea CYP6B9/H. armigera CYP6B2/Heliothis virescens CYP6B10. The low degree of divergence in the first two of these groups is comparable to allelic variation within a single species. These orthologous relationships and the high degrees of similarity in both actin and P450 genes strongly indicate that these Helicoverpa species are extremely closely related.

KW - Actin

KW - Cytochrome P450

KW - Evolution

KW - Gene conversion

KW - Heliothinae

UR - http://www.scopus.com/inward/record.url?scp=0036489169&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036489169&partnerID=8YFLogxK

U2 - 10.1016/S0965-1748(01)00092-3

DO - 10.1016/S0965-1748(01)00092-3

M3 - Article

C2 - 11804803

AN - SCOPUS:0036489169

VL - 32

SP - 311

EP - 320

JO - Insect Biochemistry and Molecular Biology

JF - Insect Biochemistry and Molecular Biology

SN - 0965-1748

IS - 3

ER -