Cytokeratin characterization of human prostatic carcinoma and its derived cell lines

Raymond B Nagle, F. R. Ahmann, K. M. McDaniel, M. L. Paquin, V. A. Clark, A. Celniker

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Two murine monoclonal anti-cytokeratin antibodies with defined specificity were shown to distinguish between basal cells and luminal cells in human prostate tissue. Forty-one biopsies or transurethral resection specimens were characterized using these two antibodies. In cases of benign prostatic hyperplasia, focal loss of the basal cell layer was noted in areas of glandular proliferation. Ten cases of adenocarcinoma of the prostate, varying in Gleason's histological grade from 2 to 4, were also studied. In each case the carcinoma was shown to represent the luminal cell phenotype with no evidence of involvement of the basal cell phenotype. An analysis of three established metastatic prostatic carcinoma cell lines (DU-145, PC-3, and LNCaP) using two-dimensional electrophoresis showed that the cytokeratin complement of each cell line was slightly different but retained the phenotype of the luminal cell. It was concluded that during both hyperplasia and neoplastic transformation of the prostate, the luminal cell phenotype is primarily involved and that the basal cell phenotype does not appear to contribute to either intraluminal proliferation or invasive cell populations.

Original languageEnglish (US)
Pages (from-to)281-286
Number of pages6
JournalCancer Research
Volume47
Issue number1
StatePublished - 1987
Externally publishedYes

Fingerprint

Keratins
Carcinoma
Cell Line
Phenotype
Prostate
Prostatic Hyperplasia
Hyperplasia
Electrophoresis
Anti-Idiotypic Antibodies
Adenocarcinoma
Monoclonal Antibodies
Biopsy
Antibodies

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Nagle, R. B., Ahmann, F. R., McDaniel, K. M., Paquin, M. L., Clark, V. A., & Celniker, A. (1987). Cytokeratin characterization of human prostatic carcinoma and its derived cell lines. Cancer Research, 47(1), 281-286.

Cytokeratin characterization of human prostatic carcinoma and its derived cell lines. / Nagle, Raymond B; Ahmann, F. R.; McDaniel, K. M.; Paquin, M. L.; Clark, V. A.; Celniker, A.

In: Cancer Research, Vol. 47, No. 1, 1987, p. 281-286.

Research output: Contribution to journalArticle

Nagle, RB, Ahmann, FR, McDaniel, KM, Paquin, ML, Clark, VA & Celniker, A 1987, 'Cytokeratin characterization of human prostatic carcinoma and its derived cell lines', Cancer Research, vol. 47, no. 1, pp. 281-286.
Nagle RB, Ahmann FR, McDaniel KM, Paquin ML, Clark VA, Celniker A. Cytokeratin characterization of human prostatic carcinoma and its derived cell lines. Cancer Research. 1987;47(1):281-286.
Nagle, Raymond B ; Ahmann, F. R. ; McDaniel, K. M. ; Paquin, M. L. ; Clark, V. A. ; Celniker, A. / Cytokeratin characterization of human prostatic carcinoma and its derived cell lines. In: Cancer Research. 1987 ; Vol. 47, No. 1. pp. 281-286.
@article{966fc9a58d364f10836ce3c9d44f1bf2,
title = "Cytokeratin characterization of human prostatic carcinoma and its derived cell lines",
abstract = "Two murine monoclonal anti-cytokeratin antibodies with defined specificity were shown to distinguish between basal cells and luminal cells in human prostate tissue. Forty-one biopsies or transurethral resection specimens were characterized using these two antibodies. In cases of benign prostatic hyperplasia, focal loss of the basal cell layer was noted in areas of glandular proliferation. Ten cases of adenocarcinoma of the prostate, varying in Gleason's histological grade from 2 to 4, were also studied. In each case the carcinoma was shown to represent the luminal cell phenotype with no evidence of involvement of the basal cell phenotype. An analysis of three established metastatic prostatic carcinoma cell lines (DU-145, PC-3, and LNCaP) using two-dimensional electrophoresis showed that the cytokeratin complement of each cell line was slightly different but retained the phenotype of the luminal cell. It was concluded that during both hyperplasia and neoplastic transformation of the prostate, the luminal cell phenotype is primarily involved and that the basal cell phenotype does not appear to contribute to either intraluminal proliferation or invasive cell populations.",
author = "Nagle, {Raymond B} and Ahmann, {F. R.} and McDaniel, {K. M.} and Paquin, {M. L.} and Clark, {V. A.} and A. Celniker",
year = "1987",
language = "English (US)",
volume = "47",
pages = "281--286",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "1",

}

TY - JOUR

T1 - Cytokeratin characterization of human prostatic carcinoma and its derived cell lines

AU - Nagle, Raymond B

AU - Ahmann, F. R.

AU - McDaniel, K. M.

AU - Paquin, M. L.

AU - Clark, V. A.

AU - Celniker, A.

PY - 1987

Y1 - 1987

N2 - Two murine monoclonal anti-cytokeratin antibodies with defined specificity were shown to distinguish between basal cells and luminal cells in human prostate tissue. Forty-one biopsies or transurethral resection specimens were characterized using these two antibodies. In cases of benign prostatic hyperplasia, focal loss of the basal cell layer was noted in areas of glandular proliferation. Ten cases of adenocarcinoma of the prostate, varying in Gleason's histological grade from 2 to 4, were also studied. In each case the carcinoma was shown to represent the luminal cell phenotype with no evidence of involvement of the basal cell phenotype. An analysis of three established metastatic prostatic carcinoma cell lines (DU-145, PC-3, and LNCaP) using two-dimensional electrophoresis showed that the cytokeratin complement of each cell line was slightly different but retained the phenotype of the luminal cell. It was concluded that during both hyperplasia and neoplastic transformation of the prostate, the luminal cell phenotype is primarily involved and that the basal cell phenotype does not appear to contribute to either intraluminal proliferation or invasive cell populations.

AB - Two murine monoclonal anti-cytokeratin antibodies with defined specificity were shown to distinguish between basal cells and luminal cells in human prostate tissue. Forty-one biopsies or transurethral resection specimens were characterized using these two antibodies. In cases of benign prostatic hyperplasia, focal loss of the basal cell layer was noted in areas of glandular proliferation. Ten cases of adenocarcinoma of the prostate, varying in Gleason's histological grade from 2 to 4, were also studied. In each case the carcinoma was shown to represent the luminal cell phenotype with no evidence of involvement of the basal cell phenotype. An analysis of three established metastatic prostatic carcinoma cell lines (DU-145, PC-3, and LNCaP) using two-dimensional electrophoresis showed that the cytokeratin complement of each cell line was slightly different but retained the phenotype of the luminal cell. It was concluded that during both hyperplasia and neoplastic transformation of the prostate, the luminal cell phenotype is primarily involved and that the basal cell phenotype does not appear to contribute to either intraluminal proliferation or invasive cell populations.

UR - http://www.scopus.com/inward/record.url?scp=0023102072&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023102072&partnerID=8YFLogxK

M3 - Article

VL - 47

SP - 281

EP - 286

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 1

ER -