Cytokine-mediated PGE2 expression in human colonic fibroblasts

Edwakd C. Kim, Yingting Zhu, Valerie Andersen, Daniela Sciaky, H. James Cao, Heather Meekins, Terry J. Smith, Michael P Lance

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

We investigated prostanoid biogenesis in human colonic fibroblasts (CCD- 18Co and 5 primary fibroblast cultures) and epithelial cell lines (NCM460, T84, HT-29, and LS 174T) and the effect of PGE2 on fibroblast morphology. Cytokine-stimulated PGE2 production was measured. PGH synthase-1 and -2 (PGHS-1 and -2) protein and mRNA expression were evaluated. Basal PGE2 levels were low in all cell types (0.15-6.47 ng/mg protein). Treatment for 24 h with interleukin-1β (IL-1β; 10 ng/ml) or tumor necrosis factor-α (50 ng/ml), respectively, elicited maximal 25- and 6-fold inductions of PGE2 synthesis in CCD-18Co cultures and similar results in primary fibroblast cultures; maximal inductions with IL-1β in colonic epithelial cell lines were from zero to fivefold. Treatment of CCD-18Co fibroblasts with IL-1β caused maximal 21- and 53-fold increases, respectively, in PGHS-2 protein and mRNA levels without altering PGHS-1 expression. PGE2 (0.1 μmol/l) elicited a dramatic shape change in selected fibroblasts. Colonic fibroblasts are potentially important as cytokine targets and a source of and target for colonic prostanoids in vivo.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume275
Issue number4 44-4
StatePublished - Oct 1998
Externally publishedYes

Fingerprint

Fibroblasts
Dinoprostone
Cytokines
Interleukin-1
Cell culture
Charge coupled devices
Cyclooxygenase 2
Prostaglandins
Epithelial Cells
Cell Line
Messenger RNA
Proteins
Prostaglandin-Endoperoxide Synthases
Interleukin-10
Tumor Necrosis Factor-alpha

Keywords

  • Colon cancer cell lines
  • Colorectal adenocarcinoma
  • Primary fibroblast cultures
  • Prostaglandin H synthases

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Kim, E. C., Zhu, Y., Andersen, V., Sciaky, D., Cao, H. J., Meekins, H., ... Lance, M. P. (1998). Cytokine-mediated PGE2 expression in human colonic fibroblasts. American Journal of Physiology - Cell Physiology, 275(4 44-4).

Cytokine-mediated PGE2 expression in human colonic fibroblasts. / Kim, Edwakd C.; Zhu, Yingting; Andersen, Valerie; Sciaky, Daniela; Cao, H. James; Meekins, Heather; Smith, Terry J.; Lance, Michael P.

In: American Journal of Physiology - Cell Physiology, Vol. 275, No. 4 44-4, 10.1998.

Research output: Contribution to journalArticle

Kim, EC, Zhu, Y, Andersen, V, Sciaky, D, Cao, HJ, Meekins, H, Smith, TJ & Lance, MP 1998, 'Cytokine-mediated PGE2 expression in human colonic fibroblasts', American Journal of Physiology - Cell Physiology, vol. 275, no. 4 44-4.
Kim EC, Zhu Y, Andersen V, Sciaky D, Cao HJ, Meekins H et al. Cytokine-mediated PGE2 expression in human colonic fibroblasts. American Journal of Physiology - Cell Physiology. 1998 Oct;275(4 44-4).
Kim, Edwakd C. ; Zhu, Yingting ; Andersen, Valerie ; Sciaky, Daniela ; Cao, H. James ; Meekins, Heather ; Smith, Terry J. ; Lance, Michael P. / Cytokine-mediated PGE2 expression in human colonic fibroblasts. In: American Journal of Physiology - Cell Physiology. 1998 ; Vol. 275, No. 4 44-4.
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