Cytokine-mediated PGE2 expression in human colonic fibroblasts

Edwakd C. Kim, Yingting Zhu, Valerie Andersen, Daniela Sciaky, H. James Cao, Heather Meekins, Terry J. Smith, Peter Lance

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45 Scopus citations

Abstract

We investigated prostanoid biogenesis in human colonic fibroblasts (CCD- 18Co and 5 primary fibroblast cultures) and epithelial cell lines (NCM460, T84, HT-29, and LS 174T) and the effect of PGE2 on fibroblast morphology. Cytokine-stimulated PGE2 production was measured. PGH synthase-1 and -2 (PGHS-1 and -2) protein and mRNA expression were evaluated. Basal PGE2 levels were low in all cell types (0.15-6.47 ng/mg protein). Treatment for 24 h with interleukin-1β (IL-1β; 10 ng/ml) or tumor necrosis factor-α (50 ng/ml), respectively, elicited maximal 25- and 6-fold inductions of PGE2 synthesis in CCD-18Co cultures and similar results in primary fibroblast cultures; maximal inductions with IL-1β in colonic epithelial cell lines were from zero to fivefold. Treatment of CCD-18Co fibroblasts with IL-1β caused maximal 21- and 53-fold increases, respectively, in PGHS-2 protein and mRNA levels without altering PGHS-1 expression. PGE2 (0.1 μmol/l) elicited a dramatic shape change in selected fibroblasts. Colonic fibroblasts are potentially important as cytokine targets and a source of and target for colonic prostanoids in vivo.

Original languageEnglish (US)
Pages (from-to)C988-C994
JournalAmerican Journal of Physiology - Cell Physiology
Volume275
Issue number4 44-4
StatePublished - Oct 1 1998
Externally publishedYes

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Keywords

  • Colon cancer cell lines
  • Colorectal adenocarcinoma
  • Primary fibroblast cultures
  • Prostaglandin H synthases

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Kim, E. C., Zhu, Y., Andersen, V., Sciaky, D., Cao, H. J., Meekins, H., Smith, T. J., & Lance, P. (1998). Cytokine-mediated PGE2 expression in human colonic fibroblasts. American Journal of Physiology - Cell Physiology, 275(4 44-4), C988-C994.