Cytokine production by peripheral blood mononuclear cells in human coccidioidomycosis

David B. Corry, Neil M. Ampel, Lara Christian, Richard M. Locksley, John N. Galgiani

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

The production and mRNA expression of the cytokines interferon-γ (IFN- γ) and interleukin (IL)-4, -10, and -12 by peripheral blood mononuclear cells (PBMC) after incubation with the coccidioidal antigen toluene spherule lysate (TSL) from various subjects were measured. The IFN-γ concentration in PBMC supernatants incubated for 72 h from 8 subjects with disseminated coccidioidomycosis was significantly less than that from 7 healthy, coccidioidal-immune subjects (P = .015). No differences were seen among the subject groups in the concentrations of IL-4, -10, or -12. By use of competitive polymerase chain reaction, PBMC from subjects with disseminated coccidioidomycosis also expressed less mRNA for IFN-γ and IL-12 than did cells from healthy, immune subjects. These data suggest that patients with disseminated coccidioidomycosis have a diminished T helper lymphocyte type 1 response.

Original languageEnglish (US)
Pages (from-to)440-443
Number of pages4
JournalJournal of Infectious Diseases
Volume174
Issue number2
DOIs
StatePublished - Jan 1 1996

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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