Cytological analysis of cytoplasmic incompatibility induced by Cardinium suggests convergent evolution with its distant cousin Wolbachia

Marco Gebiola, Massimo Giorgini, Suzanne E. Kelly, Matthew R. Doremus, Patrick M. Ferree, Martha S Hunter

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Cytoplasmic incompatibility (CI) is a conditional sterility in numerous arthropods that is caused by inherited, intracellular bacteria such as Wolbachia. Matings between males carrying CI-inducing Wolbachia and uninfected females, or between males and females infected with different Wolbachia strains, result in progeny that die during very early embryogenesis. Multiple studies in diploid (Drosophila) and haplodiploid (Nasonia) insects have shown that CI-Wolbachia cause a failure of the paternally derived chromatin from resolving into distinct chromosomes. This leads to the formation of chromatin bridges and other mitotic defects as early as the first mitotic division, and to early mitotic arrest. It is currently unknown if CIinducing symbionts other than Wolbachia affect similar cellular processes. Here, we investigated CI caused by an unrelated bacterium, Cardinium, which naturally infects a parasitic wasp, Encarsia suzannae. CI crosses in this host-symbiont system resulted in early mitotic defects including asynchrony of paternal and maternal chromosome sets as they enter mitosis, chromatin bridges and improper chromosome segregation that spanned across multiple mitotic divisions, triggering embryonic death through accumulated aneuploidy. We highlight small differences with CI-Wolbachia, which could be due to the underlying CI mechanism or host-specific effects. Our results suggest a convergence of CI-related cellular phenotypes between these two unrelated symbionts.

Original languageEnglish (US)
Article number20171433
JournalProceedings of the Royal Society B: Biological Sciences
Volume284
Issue number1862
DOIs
StatePublished - Sep 13 2017

Fingerprint

cytoplasmic incompatibility
Wolbachia
convergent evolution
Chromosomes
Chromatin
Bacteria
Defects
symbiont
symbionts
chromatin
chromosome
defect
Wasps
Chromosome Segregation
Arthropods
Nasonia
Aneuploidy
Encarsia
chromosomes
Diploidy

Keywords

  • Bacterial endosymbionts
  • Embryonic death
  • Mitotic defects
  • Parasitoid
  • Reproductive manipulators

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

Cite this

Cytological analysis of cytoplasmic incompatibility induced by Cardinium suggests convergent evolution with its distant cousin Wolbachia. / Gebiola, Marco; Giorgini, Massimo; Kelly, Suzanne E.; Doremus, Matthew R.; Ferree, Patrick M.; Hunter, Martha S.

In: Proceedings of the Royal Society B: Biological Sciences, Vol. 284, No. 1862, 20171433, 13.09.2017.

Research output: Contribution to journalArticle

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