Cytology of cobalt-filled neurons in flies

cobalt deposits at presynaptic and postsynaptic sites, mitochondria and the cytoskeleton

U. K. Bassemir, Nicholas J Strausfeld

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Combined light and electron microscopy of identified neurons requires an intracellular marker that is both photon opaque and has electron scattering properties. We describe results using cobalt chloride block intensified with silver as an intracellular label. The novelty of the method is its integration in tissue fixation, prior to dehydration, resulting in fine grain precipitates that resolve certain intracellular structures. Filled neurons are clearly distinguishable from unfilled profiles by cobalt-silver precipitates. Energy dispersive X-ray analysis confirms that silver is specifically deposited onto cobalt sulphide cores which are characteristically associated with microtubules, mitochondria, presynaptic and postsynaptic specializations and gap junction-like membrane appositions.

Original languageEnglish (US)
Pages (from-to)949-970
Number of pages22
JournalJournal of Neurocytology
Volume12
Issue number6
DOIs
StatePublished - Dec 1983
Externally publishedYes

Fingerprint

Cobalt
Cytoskeleton
Silver
Diptera
Cell Biology
Mitochondria
Neurons
Tissue Fixation
Gap Junctions
Photons
Dehydration
Microtubules
Electron Microscopy
X-Rays
Electrons
Light
Membranes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Histology
  • Anatomy
  • Cell Biology

Cite this

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T1 - Cytology of cobalt-filled neurons in flies

T2 - cobalt deposits at presynaptic and postsynaptic sites, mitochondria and the cytoskeleton

AU - Bassemir, U. K.

AU - Strausfeld, Nicholas J

PY - 1983/12

Y1 - 1983/12

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AB - Combined light and electron microscopy of identified neurons requires an intracellular marker that is both photon opaque and has electron scattering properties. We describe results using cobalt chloride block intensified with silver as an intracellular label. The novelty of the method is its integration in tissue fixation, prior to dehydration, resulting in fine grain precipitates that resolve certain intracellular structures. Filled neurons are clearly distinguishable from unfilled profiles by cobalt-silver precipitates. Energy dispersive X-ray analysis confirms that silver is specifically deposited onto cobalt sulphide cores which are characteristically associated with microtubules, mitochondria, presynaptic and postsynaptic specializations and gap junction-like membrane appositions.

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