Cytoplasmic dyein is required for poleward chromosome movement during mitosis in Drosophila embryos

D. J. Sharp, Gregory C. Rogers, J. M. Scholey

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

The movement of chromosomes during mitosis occurs on a bipolar, microtubule-based protein machine, the mitotic spindle. It has long been proposed that poleward chromosome movements that occur during prometaphase and anaphase A are driven by the microtubule motor cytoplasmic dynein, which binds to kinetochores and transports them toward the minus ends of spindle microtubules. Here we evaluate this hypothesis using time-lapse confocal microscopy to visualize, in real time, kinetochore and chromatid movements in living Drosophila embryos in the presence and absence of specific inhibitors of cytoplasmic dynein. Our results show that dynein inhibitors disrupt the alignment of kinetochores on the metaphase spindle equator and also interfere with kinetochore- and chromatidto-pole movements during anaphase A. Thus, dyein is essential for poleward chromosome motility throughout mitosis in Drosophila embryos.

Original languageEnglish (US)
Pages (from-to)922-930
Number of pages9
JournalNature Cell Biology
Volume2
Issue number12
DOIs
StatePublished - 2000
Externally publishedYes

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Kinetochores
Mitosis
Drosophila
Embryonic Structures
Chromosomes
Cytoplasmic Dyneins
Anaphase
Microtubules
Prometaphase
Microtubule Proteins
Dyneins
Spindle Apparatus
Chromatids
Metaphase
Confocal Microscopy

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cytoplasmic dyein is required for poleward chromosome movement during mitosis in Drosophila embryos. / Sharp, D. J.; Rogers, Gregory C.; Scholey, J. M.

In: Nature Cell Biology, Vol. 2, No. 12, 2000, p. 922-930.

Research output: Contribution to journalArticle

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