Cytoplasmic incompatibility and multiple symbiont infection in the ash whitefly parasitoid, Encarsia inaron

Steve J. Perlman, Suzanne E. Kelly, Einat Zchori-Fein, Martha S Hunter

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Terrestrial arthropods are commonly infected with maternally inherited symbionts that cause reproductive incompatibilities between hosts with differing infection status. Such symbionts can have major effects on the efficacy of a biological control program if releases are comprised of mixtures of differentially infected individuals. In this study, the ash whitefly parasitoid, Encarsia inaron (Hymenoptera: Aphelinidae) from Arizona was surveyed for the presence of heritable bacterial symbionts; experiments were also performed to test for two phenotypes known to be caused by Encarsia symbionts-cytoplasmic incompatibility and changes in oviposition behavior and host use. E. inaron has successfully reduced ash whitefly to non-pest status in all three locations it has been released (California, Arizona, and North Carolina) and is also notable as one of the only Encarsia species that is not autoparasitic, with both male and female wasps developing as primary parasitoids of whiteflies. We show that E. inaron is infected with both Wolbachia and Cardinium. While there was no effect of the symbionts on oviposition behavior or host use, crosses between doubly infected male wasps and uninfected females resulted in a severe reduction in the number of female offspring; male offspring production was unaffected. This study thus serves as a further warning that ascribing a phenotype to a symbiont with confidence depends on eliminating the possibility of a mixed infection, and establishes E. inaron as a useful model for dissecting Wolbachia-Cardinium interactions.

Original languageEnglish (US)
Pages (from-to)474-480
Number of pages7
JournalBiological Control
Volume39
Issue number3
DOIs
StatePublished - Dec 2006

Fingerprint

Siphoninus phillyreae
cytoplasmic incompatibility
Encarsia
whitefly
symbiont
parasitoid
symbionts
ash
infection
host use
Wolbachia
wasp
oviposition
phenotype
Aphelinidae
incompatibility
Aleyrodidae
mixed infection
biological control
arthropod

Keywords

  • Biological control
  • Cardinium
  • Female mortality
  • Reproductive manipulation
  • Vertical transmission
  • Wolbachia

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Ecology, Evolution, Behavior and Systematics

Cite this

Cytoplasmic incompatibility and multiple symbiont infection in the ash whitefly parasitoid, Encarsia inaron. / Perlman, Steve J.; Kelly, Suzanne E.; Zchori-Fein, Einat; Hunter, Martha S.

In: Biological Control, Vol. 39, No. 3, 12.2006, p. 474-480.

Research output: Contribution to journalArticle

Perlman, Steve J. ; Kelly, Suzanne E. ; Zchori-Fein, Einat ; Hunter, Martha S. / Cytoplasmic incompatibility and multiple symbiont infection in the ash whitefly parasitoid, Encarsia inaron. In: Biological Control. 2006 ; Vol. 39, No. 3. pp. 474-480.
@article{7ef1b29336764a95b09c4e90423e5965,
title = "Cytoplasmic incompatibility and multiple symbiont infection in the ash whitefly parasitoid, Encarsia inaron",
abstract = "Terrestrial arthropods are commonly infected with maternally inherited symbionts that cause reproductive incompatibilities between hosts with differing infection status. Such symbionts can have major effects on the efficacy of a biological control program if releases are comprised of mixtures of differentially infected individuals. In this study, the ash whitefly parasitoid, Encarsia inaron (Hymenoptera: Aphelinidae) from Arizona was surveyed for the presence of heritable bacterial symbionts; experiments were also performed to test for two phenotypes known to be caused by Encarsia symbionts-cytoplasmic incompatibility and changes in oviposition behavior and host use. E. inaron has successfully reduced ash whitefly to non-pest status in all three locations it has been released (California, Arizona, and North Carolina) and is also notable as one of the only Encarsia species that is not autoparasitic, with both male and female wasps developing as primary parasitoids of whiteflies. We show that E. inaron is infected with both Wolbachia and Cardinium. While there was no effect of the symbionts on oviposition behavior or host use, crosses between doubly infected male wasps and uninfected females resulted in a severe reduction in the number of female offspring; male offspring production was unaffected. This study thus serves as a further warning that ascribing a phenotype to a symbiont with confidence depends on eliminating the possibility of a mixed infection, and establishes E. inaron as a useful model for dissecting Wolbachia-Cardinium interactions.",
keywords = "Biological control, Cardinium, Female mortality, Reproductive manipulation, Vertical transmission, Wolbachia",
author = "Perlman, {Steve J.} and Kelly, {Suzanne E.} and Einat Zchori-Fein and Hunter, {Martha S}",
year = "2006",
month = "12",
doi = "10.1016/j.biocontrol.2006.05.015",
language = "English (US)",
volume = "39",
pages = "474--480",
journal = "Biological Control",
issn = "1049-9644",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Cytoplasmic incompatibility and multiple symbiont infection in the ash whitefly parasitoid, Encarsia inaron

AU - Perlman, Steve J.

AU - Kelly, Suzanne E.

AU - Zchori-Fein, Einat

AU - Hunter, Martha S

PY - 2006/12

Y1 - 2006/12

N2 - Terrestrial arthropods are commonly infected with maternally inherited symbionts that cause reproductive incompatibilities between hosts with differing infection status. Such symbionts can have major effects on the efficacy of a biological control program if releases are comprised of mixtures of differentially infected individuals. In this study, the ash whitefly parasitoid, Encarsia inaron (Hymenoptera: Aphelinidae) from Arizona was surveyed for the presence of heritable bacterial symbionts; experiments were also performed to test for two phenotypes known to be caused by Encarsia symbionts-cytoplasmic incompatibility and changes in oviposition behavior and host use. E. inaron has successfully reduced ash whitefly to non-pest status in all three locations it has been released (California, Arizona, and North Carolina) and is also notable as one of the only Encarsia species that is not autoparasitic, with both male and female wasps developing as primary parasitoids of whiteflies. We show that E. inaron is infected with both Wolbachia and Cardinium. While there was no effect of the symbionts on oviposition behavior or host use, crosses between doubly infected male wasps and uninfected females resulted in a severe reduction in the number of female offspring; male offspring production was unaffected. This study thus serves as a further warning that ascribing a phenotype to a symbiont with confidence depends on eliminating the possibility of a mixed infection, and establishes E. inaron as a useful model for dissecting Wolbachia-Cardinium interactions.

AB - Terrestrial arthropods are commonly infected with maternally inherited symbionts that cause reproductive incompatibilities between hosts with differing infection status. Such symbionts can have major effects on the efficacy of a biological control program if releases are comprised of mixtures of differentially infected individuals. In this study, the ash whitefly parasitoid, Encarsia inaron (Hymenoptera: Aphelinidae) from Arizona was surveyed for the presence of heritable bacterial symbionts; experiments were also performed to test for two phenotypes known to be caused by Encarsia symbionts-cytoplasmic incompatibility and changes in oviposition behavior and host use. E. inaron has successfully reduced ash whitefly to non-pest status in all three locations it has been released (California, Arizona, and North Carolina) and is also notable as one of the only Encarsia species that is not autoparasitic, with both male and female wasps developing as primary parasitoids of whiteflies. We show that E. inaron is infected with both Wolbachia and Cardinium. While there was no effect of the symbionts on oviposition behavior or host use, crosses between doubly infected male wasps and uninfected females resulted in a severe reduction in the number of female offspring; male offspring production was unaffected. This study thus serves as a further warning that ascribing a phenotype to a symbiont with confidence depends on eliminating the possibility of a mixed infection, and establishes E. inaron as a useful model for dissecting Wolbachia-Cardinium interactions.

KW - Biological control

KW - Cardinium

KW - Female mortality

KW - Reproductive manipulation

KW - Vertical transmission

KW - Wolbachia

UR - http://www.scopus.com/inward/record.url?scp=33750468633&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750468633&partnerID=8YFLogxK

U2 - 10.1016/j.biocontrol.2006.05.015

DO - 10.1016/j.biocontrol.2006.05.015

M3 - Article

AN - SCOPUS:33750468633

VL - 39

SP - 474

EP - 480

JO - Biological Control

JF - Biological Control

SN - 1049-9644

IS - 3

ER -