Cytoplasmic microinjection of immunoglobulin Gs recognizing RNA helices inhibits human cell growth

David A. Zarling, Cornelia J. Calhoun, Burt G.F. Feuerstein, Elissa P. Sena

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We report here that nucleolar and cytoplasmic RNA in mammalian cells is recognized specifically by both experimentally induced monoclonal IgG unique for left-handed Z-RNA and by autoimmune mouse monoclonal IgG specific for ribosomal RNA. Nucleolar Z-RNA synthesis, like nucleolar ribosomal RNA synthesis, is inhibited by actinomycin D treatment and dimethylsulfoxide-induced differentiation. Immune anti-Z-RNA IgGs microinjected into living nuclei bind nucleolar RNA, and these complexes appear to be removed from the nucleus within minutes. Cytoplasmically microinjected monoclonal or polyclonal anti-Z-RNA IgGs specifically bind cytoplasmic RNA and inhibit cell multiplication. Microinjection of antibodies directed against double-stranded A-form RNA also inhibits cell growth, indicating physiological roles for both these double-stranded RNAs. Elevated ionic conditions, which in energy-minimized models can cause the walls of the groove in Z-RNA (but not Z-DNA) to approach each other and close, also prevent antibody binding to specific synthetic or cellular Z-RNA determinants. Our antibodies binding unique Z-RNA structures probably recognize antigens determined by the exposed 2′-OH ribose sugarphosphate groups.

Original languageEnglish (US)
Pages (from-to)147-160
Number of pages14
JournalJournal of Molecular Biology
Volume211
Issue number1
DOIs
StatePublished - Jan 5 1990
Externally publishedYes

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Microinjections
Immunoglobulins
RNA
Growth
Ribosomal RNA
Antibodies
Immunoglobulin G
Z-Form DNA
Ribose
Double-Stranded RNA
Dactinomycin
Dimethyl Sulfoxide
Cell Proliferation
Antigens

ASJC Scopus subject areas

  • Virology

Cite this

Cytoplasmic microinjection of immunoglobulin Gs recognizing RNA helices inhibits human cell growth. / Zarling, David A.; Calhoun, Cornelia J.; Feuerstein, Burt G.F.; Sena, Elissa P.

In: Journal of Molecular Biology, Vol. 211, No. 1, 05.01.1990, p. 147-160.

Research output: Contribution to journalArticle

Zarling, David A. ; Calhoun, Cornelia J. ; Feuerstein, Burt G.F. ; Sena, Elissa P. / Cytoplasmic microinjection of immunoglobulin Gs recognizing RNA helices inhibits human cell growth. In: Journal of Molecular Biology. 1990 ; Vol. 211, No. 1. pp. 147-160.
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