Cytotoxic and antihaptotactic beauvericin analogues from precursor-directed biosynthesis with the insect pathogen Beauveria bassiana ATCC 7159

Yuquan Xu, Jixun Zhan, E. M Kithsiri Wijeratne, Anna M. Burns, Leslie Gunatilaka, Istvan Molnar

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Precursor-directed biosynthesis was used to produce analogues of the cyclic depsipeptide mycotoxin beauvericin (1) using the filamentous fungus Beauveria bassiana ATCC 7159. Feeding 30 analogues of D-2-hydroxyisovalerate and L-phenylalanine, the natural 2-hydroxycarboxylic acid and amino acid precursors of beauvericin, led to the biosynthesis of novel beauvericins. Six of these were isolated and characterized, and their cytotoxicity and directional cell migration (haptotaxis) inhibitory activity against the metastatic prostate cancer cell line PC-3M were evaluated. Replacement of one, two, or all three of the D-2-hydroxyisovalerate constituents in beauvericin (1) with 2-hydroxybutyrate moieties (beauvericins G1-3, compounds 2-4) caused a parallel decline of cell migration inhibitory activity and cytotoxicity, suggesting a requirement for a branched side chain for both of these biological activities at the corresponding positions of beauvericins. Replacement of one, two, or all three N-methyl-L-phenylalanine residues of beauvericin with N-methyl-L-3-fluorophenylalanine moieties (beauvericins H1-3, compounds 5-7) increased cytotoxicity without affecting antihaptotactic activity.

Original languageEnglish (US)
Pages (from-to)1467-1471
Number of pages5
JournalJournal of Natural Products
Volume70
Issue number9
DOIs
StatePublished - Sep 2007

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Beauveria
entomopathogens
Biosynthesis
Beauveria bassiana
Pathogens
Insects
cytotoxicity
biosynthesis
cell movement
phenylalanine
depsipeptides
Cytotoxicity
prostatic neoplasms
mycotoxins
bioactive properties
Phenylalanine
cell lines
Cell Movement
amino acids
fungi

ASJC Scopus subject areas

  • Plant Science
  • Chemistry (miscellaneous)
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Cytotoxic and antihaptotactic beauvericin analogues from precursor-directed biosynthesis with the insect pathogen Beauveria bassiana ATCC 7159. / Xu, Yuquan; Zhan, Jixun; Wijeratne, E. M Kithsiri; Burns, Anna M.; Gunatilaka, Leslie; Molnar, Istvan.

In: Journal of Natural Products, Vol. 70, No. 9, 09.2007, p. 1467-1471.

Research output: Contribution to journalArticle

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