Cytotoxic response profiles of cultured renal epithelial and mesenchymal cells to selected aromatic hydrocarbons

A. R. Parrish, N. F. Alejandro, R. C. Bowes, Kenneth Ramos

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The cytotoxic responses of cultured rat renal cortico-tubular epithelial cells (TECs) and glomerular mesangial cells (GMCs) to selected polycyclic and polyhalogenated aromatic hydrocarbons (AHs) were evaluated to further define class specific differences in toxicity potential among these chemicals. Primary cultures of renal cells were exposed to benzo[a]pyrene (BaP, 0.3-30 μM); naphthalene (NAPH, 1-1000 μM); 2-methylnaphthalene (2-MNAPH, 1-1000 μM), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, 0.01-1 nM); 2,3,7,8- tetrachlorodibenzofuran (TCDF, 0.05-5 nM; and pentachlorophenol (PCP, 0.1-10 μM for 4 or 24 hr. Measurements of mitochondrial membrane fragility in TECs revealed that the polycyclic aromatic hydrocarbons (PAHs) (BaP, NAPH and 2- MNAPH) preferentially injured these cells relative to halogenated aromatic hydrocarbons (HAHs). Extended exposures to all AHs tested were associated with moderate mitochondrial injury in TECs. Challenge of TECs with AHs for 4 hr did not modulate GSH levels, although modest increases in this tripeptide occurred on extended exposures. Similar response profiles were observed in GMCs, where PAHs elicited mitochondrial damage by 4 hr, while extended challenge was associated with injury in response to all AHS. Only aryl hydrocarbon receptor (AhR) ligands (BaP, TCDD and TCDF) depleted intracellular glutathione (GSH) in GMCs, while extended exposures to BaP and TCDD, as well as NAPH and 2-MNAPH, were associated with rebound increases in cellular GSH content. These data indicate that AHs can compromise renal cell function by interference with mitochondrial function and GSH homeostasis and implicate both epithelial and mesenchymal populations in the nephrotoxic response to this heterogeneous class of chemicals.

Original languageEnglish (US)
Pages (from-to)219-232
Number of pages14
JournalToxicology in Vitro
Volume12
Issue number3
DOIs
StatePublished - Jun 1 1998
Externally publishedYes

Fingerprint

Aromatic Hydrocarbons
Mesangial Cells
Polycyclic Aromatic Hydrocarbons
Epithelial Cells
Kidney
Halogenated Hydrocarbons
Pentachlorophenol
Aryl Hydrocarbon Receptors
Primary Cell Culture
Benzo(a)pyrene
Wounds and Injuries
Mitochondrial Membranes
Glutathione
Toxicity
Rats
Homeostasis
Ligands
Membranes
Polychlorinated Dibenzodioxins
Population

Keywords

  • Aromatic hydrocarbons
  • Glomerural mesangial cells
  • Glutathione
  • Mitochondrial membrane fragility
  • Tubular epithelial cells

ASJC Scopus subject areas

  • Toxicology

Cite this

Cytotoxic response profiles of cultured renal epithelial and mesenchymal cells to selected aromatic hydrocarbons. / Parrish, A. R.; Alejandro, N. F.; Bowes, R. C.; Ramos, Kenneth.

In: Toxicology in Vitro, Vol. 12, No. 3, 01.06.1998, p. 219-232.

Research output: Contribution to journalArticle

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