Cytotoxic T lymphocytes recognize determinants on the BALB/c-H-2Ld molecule controlled by α1 and α2 but not α3 external domains

Iwona Stroynowski, Anders Orn, Robert S. Goodenow, Minnie McMillan, James Forman, Peter R. Brayton, Jeffrey Frelingers, Leroy Hood

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

We have shown that cytotoxic T lymphocytes (CTL) raised in H-2dmice use H-2Ld but not H-2Dd or H-2Kd antigens as restricting elements in lymphocytic choriomeningitis virus (LCMV) and vesicular stomatis virus (VSV) infections. To localize the regions of H-2Ld protein recognized by CTL, we constructed a recombinant H-2Ld/Ddgene encoding a hybrid antigen with α1 and α2 external domains of H-2Ld and α3, transmembrane and cytoplasmic domains of H-2Dd. The recombinant gene was transfected into mouse cells and the hybrid molecules were characterized serologically, biochemically and functionally. In all assays, H-2Ld/Dd molecules were recognized by LCMV- and VSV-specific H-2Ld-restricted CTL in a manner similar to that of wild-type H-2Ld antigens. Analogous results were obtained with alloreactive CTL. Hybrid antigens containing the α3 domain of H-2Ld fused to α1 and α2 domains of a Qa-2,3 region-encoded antigen were not used as restricting elements by LCMV-specific CTL. These results suggest that H-2Ld-restricted CTL directed against LCMV and VSV recognize determinants controlled by the α1 and/or α2 domains of the H-2Ld molecule.

Original languageEnglish (US)
Pages (from-to)141-154
Number of pages14
JournalImmunogenetics
Volume20
Issue number2
DOIs
StatePublished - Aug 1984

ASJC Scopus subject areas

  • Immunology
  • Genetics

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