Cytotoxicity and binding profiles of activated Cry1Ac and Cry2Ab to three insect cell lines

Jizhen Wei, Gemei Liang, Kongming Wu, Shaohua Gu, Yuyuan Guo, Xinzhi Ni, Xianchun Li

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

While Cry1Ac has been known to bind with larval midgut proteins cadherin, APN (amino peptidase N), ALP (alkaline phosphatase) and ABCC2 (adenosine triphosphate-binding cassette transporter subfamily C2), little is known about the receptors of Cry2Ab. To provide a clue to the receptors of Cry2Ab, we tested the baseline cytotoxicity of activated Cry1Ac and Cry2Ab against the midgut and fat body cell lines of Helicoverpa zea and the ovary cell line of Spodoptera frugiperda (SF9). As expected, the descending order of cytotoxicity of Cry1Ac against the three cell lines in terms of 50% lethal concetration (LC50) was midgut (31.0 μg/mL) > fat body (59.0 μg/mL) and SF9 cell (99.6 μg/mL). By contrast, the fat body cell line (LC50 = 7.55 μg/mL) was about twice more susceptible to Cry2Ab than the midgut cell line (16.0 μg/mL), the susceptibility of which was not significantly greater than that of SF9 cells (27.0 μg/mL). Further, ligand blot showed the binding differences between Cry1Ac and Cry2Ab in the three cell lines. These results indicated that the receptors of Cry2Ab were enriched in fat body cells and thus largely different from the receptors of Cry1Ac, which were enriched in midgut cells.

Original languageEnglish (US)
Pages (from-to)655-666
Number of pages12
JournalInsect Science
Volume25
Issue number4
DOIs
StatePublished - Aug 2018

Keywords

  • Bt
  • SF9 cell
  • cell toxicity
  • fat body cell
  • midgut cell
  • receptors

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Agronomy and Crop Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Insect Science

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