DAG Lipase Activity Is Necessary for TRP Channel Regulation in Drosophila Photoreceptors

Hung Tat Leung, Julie Tseng-Crank, Eunju Kim, Cecon Mahapatra, Shikoh Shino, Ying Zhou, Lingling An, Rebecca W. Doerge, William L. Pak

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

In Drosophila, a phospholipase C-mediated signaling cascade links photoexcitation of rhodopsin to the opening of the TRP/TRPL channels. A lipid product of the cascade, diacylglycerol (DAG) and its metabolite(s), polyunsaturated fatty acids (PUFAs), have both been proposed as potential excitatory messengers. A crucial enzyme in the understanding of this process is likely to be DAG lipase (DAGL). However, DAGLs that might fulfill this role have not been previously identified in any organism. In this work, the Drosophila DAGL gene, inaE, has been identified from mutants that are defective in photoreceptor responses to light. The inaE-encoded protein isoforms show high sequence similarity to known mammalian DAG lipases, exhibit DAG lipase activity in vitro, and are highly expressed in photoreceptors. Analyses of norpA inaE double mutants and severe inaE mutants show that normal DAGL activity is required for the generation of physiologically meaningful photoreceptor responses.

Original languageEnglish (US)
Pages (from-to)884-896
Number of pages13
JournalNeuron
Volume58
Issue number6
DOIs
StatePublished - Jun 26 2008
Externally publishedYes

Fingerprint

Lipoprotein Lipase
Drosophila
Lipase
Rhodopsin
Diglycerides
Type C Phospholipases
Unsaturated Fatty Acids
Protein Isoforms
Lipids
Light
Enzymes
Genes

Keywords

  • CELLBIO
  • SIGNALING
  • SYSNEURO

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Leung, H. T., Tseng-Crank, J., Kim, E., Mahapatra, C., Shino, S., Zhou, Y., ... Pak, W. L. (2008). DAG Lipase Activity Is Necessary for TRP Channel Regulation in Drosophila Photoreceptors. Neuron, 58(6), 884-896. https://doi.org/10.1016/j.neuron.2008.05.001

DAG Lipase Activity Is Necessary for TRP Channel Regulation in Drosophila Photoreceptors. / Leung, Hung Tat; Tseng-Crank, Julie; Kim, Eunju; Mahapatra, Cecon; Shino, Shikoh; Zhou, Ying; An, Lingling; Doerge, Rebecca W.; Pak, William L.

In: Neuron, Vol. 58, No. 6, 26.06.2008, p. 884-896.

Research output: Contribution to journalArticle

Leung, HT, Tseng-Crank, J, Kim, E, Mahapatra, C, Shino, S, Zhou, Y, An, L, Doerge, RW & Pak, WL 2008, 'DAG Lipase Activity Is Necessary for TRP Channel Regulation in Drosophila Photoreceptors', Neuron, vol. 58, no. 6, pp. 884-896. https://doi.org/10.1016/j.neuron.2008.05.001
Leung HT, Tseng-Crank J, Kim E, Mahapatra C, Shino S, Zhou Y et al. DAG Lipase Activity Is Necessary for TRP Channel Regulation in Drosophila Photoreceptors. Neuron. 2008 Jun 26;58(6):884-896. https://doi.org/10.1016/j.neuron.2008.05.001
Leung, Hung Tat ; Tseng-Crank, Julie ; Kim, Eunju ; Mahapatra, Cecon ; Shino, Shikoh ; Zhou, Ying ; An, Lingling ; Doerge, Rebecca W. ; Pak, William L. / DAG Lipase Activity Is Necessary for TRP Channel Regulation in Drosophila Photoreceptors. In: Neuron. 2008 ; Vol. 58, No. 6. pp. 884-896.
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