De novo next generation sequencing of plant genomes

Steve Rounsley, Pradeep Reddy Marri, Yeisoo Yu, Ruifeng He, Nick Sisneros, Jose Luis Goicoechea, So Jeong Lee, Angelina Angelova, Dave Kudrna, Meizhong Luo, Jason Affourtit, Brian Desany, James Knight, Faheem Niazi, Michael Egholm, Rod A. Wing

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

The genome sequencing of all major food and bioenergy crops is of critical importance in the race to improve crop production to meet the future food and energy security needs of the world. Next generation sequencing technologies have brought about great improvements in sequencing throughput and cost, but do not yet allow for de novo sequencing of large repetitive genomes as found in most crop plants.We present a strategy that combines cutting edge next generation sequencing with "old school" genomics resources and allows rapid cost-effective sequencing of plant genomes.

Original languageEnglish (US)
Pages (from-to)35-43
Number of pages9
JournalRice
Volume2
Issue number1
DOIs
StatePublished - Mar 2009

Keywords

  • Genome assembly
  • Genome sequencing
  • Next generation sequencing
  • Oryza

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science
  • Plant Science

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