Decreased 5' nucleotidase activity in lymphocytes from asymptomatic sexually active homosexual men and patients with the acquired immune deficiency syndrome

J. L. Murray, J. M. Reuben, C. G. Munn, P. W. Mansell, G. R. Newell, Evan M Hersh

Research output: Contribution to journalArticle

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Abstract

5' Nucleotidase (5'NT) is an ectoenzyme associated with the plasma membrane of most mammalian cells. Low 5'NT activity has been observed in peripheral blood lymphocytes from patients with immunodeficiency states. 5'NT activity was measured in null and T-enriched lymphocytes from asymptomatic homosexual men and from 20 men with various degrees of the acquired immune deficiency syndrome (AIDS). Asymptomatic homosexuals were self-referred because of their concern about AIDS and were not necessarily representative of homosexuals in the general population. Enzyme activity was significantly decreased in both null (7.0 ± 2.4 nmol/106 cells/h) and T-enriched (12.0 ± 6.0 nmol/106 cells/h) lymphocytes in homosexuals as compared to lymphocytes from aged-matched heterosexual male and female controls (null = 10.8 ± 6.5 and T = 22.3 ± 10.6, P < .0001 and .008, respectively). Decreased activity was present regardless of whether the patients were asymptomatic, had prodromal symptoms such as fever, lymph node enlargement, weight loss and diarrhea, or had opportunistic infections or Kaposi's sarcoma. Homosexuals had a significantly higher fraction of lymphocytes expressing the activation antigens T10 (20% ± 3.3%) and Ia (13% ± 2.9%) than controls (11% ± 1.8% and 5% ± 0.8%, respectively, P < .05). They also had a significantly lower fraction of OKT4-positive helper lymphocytes than controls (22% ± 3.4% v 35% ± 2.2%, P < .05). 5'NT activity in lymphocytes enriched for null cells from homosexuals correlated inversely with the percentage of Ia-positive lymphocytes (r = -.655; P < .02). There was no correlation between 5'NT activity and the percentage of T4- or T8-positive lymphocytes or the T4/T8 ratio. Moreover, 5'NT activity was significantly decreased in both OKT4 (P < .025) and OKT8 (P < .05) enriched lymphocytes in homosexuals compared to controls. The data suggest that decreases in 5'NT may be a generalized defect of the peripheral blood T lymphocytes from active homosexuals that is independent of increases or decreases in specific T subpopulations or clinical status. It may contribute to the pathogenesis of AIDS.

Original languageEnglish (US)
Pages (from-to)1016-1021
Number of pages6
JournalBlood
Volume64
Issue number5
StatePublished - 1984
Externally publishedYes

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5'-Nucleotidase
Lymphocytes
Acquired Immunodeficiency Syndrome
CD38 Antigens
CD8-Positive T-Lymphocytes
Prodromal Symptoms
Blood
Null Lymphocytes
CD4-CD8 Ratio
Sexual Minorities
Kaposi's Sarcoma
Heterosexuality
Opportunistic Infections
Lymphocyte Activation
T-cells
Enzyme activity
Weight Loss
Diarrhea
Cell membranes
Fever

ASJC Scopus subject areas

  • Hematology

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Decreased 5' nucleotidase activity in lymphocytes from asymptomatic sexually active homosexual men and patients with the acquired immune deficiency syndrome. / Murray, J. L.; Reuben, J. M.; Munn, C. G.; Mansell, P. W.; Newell, G. R.; Hersh, Evan M.

In: Blood, Vol. 64, No. 5, 1984, p. 1016-1021.

Research output: Contribution to journalArticle

Murray, J. L. ; Reuben, J. M. ; Munn, C. G. ; Mansell, P. W. ; Newell, G. R. ; Hersh, Evan M. / Decreased 5' nucleotidase activity in lymphocytes from asymptomatic sexually active homosexual men and patients with the acquired immune deficiency syndrome. In: Blood. 1984 ; Vol. 64, No. 5. pp. 1016-1021.
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abstract = "5' Nucleotidase (5'NT) is an ectoenzyme associated with the plasma membrane of most mammalian cells. Low 5'NT activity has been observed in peripheral blood lymphocytes from patients with immunodeficiency states. 5'NT activity was measured in null and T-enriched lymphocytes from asymptomatic homosexual men and from 20 men with various degrees of the acquired immune deficiency syndrome (AIDS). Asymptomatic homosexuals were self-referred because of their concern about AIDS and were not necessarily representative of homosexuals in the general population. Enzyme activity was significantly decreased in both null (7.0 ± 2.4 nmol/106 cells/h) and T-enriched (12.0 ± 6.0 nmol/106 cells/h) lymphocytes in homosexuals as compared to lymphocytes from aged-matched heterosexual male and female controls (null = 10.8 ± 6.5 and T = 22.3 ± 10.6, P < .0001 and .008, respectively). Decreased activity was present regardless of whether the patients were asymptomatic, had prodromal symptoms such as fever, lymph node enlargement, weight loss and diarrhea, or had opportunistic infections or Kaposi's sarcoma. Homosexuals had a significantly higher fraction of lymphocytes expressing the activation antigens T10 (20{\%} ± 3.3{\%}) and Ia (13{\%} ± 2.9{\%}) than controls (11{\%} ± 1.8{\%} and 5{\%} ± 0.8{\%}, respectively, P < .05). They also had a significantly lower fraction of OKT4-positive helper lymphocytes than controls (22{\%} ± 3.4{\%} v 35{\%} ± 2.2{\%}, P < .05). 5'NT activity in lymphocytes enriched for null cells from homosexuals correlated inversely with the percentage of Ia-positive lymphocytes (r = -.655; P < .02). There was no correlation between 5'NT activity and the percentage of T4- or T8-positive lymphocytes or the T4/T8 ratio. Moreover, 5'NT activity was significantly decreased in both OKT4 (P < .025) and OKT8 (P < .05) enriched lymphocytes in homosexuals compared to controls. The data suggest that decreases in 5'NT may be a generalized defect of the peripheral blood T lymphocytes from active homosexuals that is independent of increases or decreases in specific T subpopulations or clinical status. It may contribute to the pathogenesis of AIDS.",
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T1 - Decreased 5' nucleotidase activity in lymphocytes from asymptomatic sexually active homosexual men and patients with the acquired immune deficiency syndrome

AU - Murray, J. L.

AU - Reuben, J. M.

AU - Munn, C. G.

AU - Mansell, P. W.

AU - Newell, G. R.

AU - Hersh, Evan M

PY - 1984

Y1 - 1984

N2 - 5' Nucleotidase (5'NT) is an ectoenzyme associated with the plasma membrane of most mammalian cells. Low 5'NT activity has been observed in peripheral blood lymphocytes from patients with immunodeficiency states. 5'NT activity was measured in null and T-enriched lymphocytes from asymptomatic homosexual men and from 20 men with various degrees of the acquired immune deficiency syndrome (AIDS). Asymptomatic homosexuals were self-referred because of their concern about AIDS and were not necessarily representative of homosexuals in the general population. Enzyme activity was significantly decreased in both null (7.0 ± 2.4 nmol/106 cells/h) and T-enriched (12.0 ± 6.0 nmol/106 cells/h) lymphocytes in homosexuals as compared to lymphocytes from aged-matched heterosexual male and female controls (null = 10.8 ± 6.5 and T = 22.3 ± 10.6, P < .0001 and .008, respectively). Decreased activity was present regardless of whether the patients were asymptomatic, had prodromal symptoms such as fever, lymph node enlargement, weight loss and diarrhea, or had opportunistic infections or Kaposi's sarcoma. Homosexuals had a significantly higher fraction of lymphocytes expressing the activation antigens T10 (20% ± 3.3%) and Ia (13% ± 2.9%) than controls (11% ± 1.8% and 5% ± 0.8%, respectively, P < .05). They also had a significantly lower fraction of OKT4-positive helper lymphocytes than controls (22% ± 3.4% v 35% ± 2.2%, P < .05). 5'NT activity in lymphocytes enriched for null cells from homosexuals correlated inversely with the percentage of Ia-positive lymphocytes (r = -.655; P < .02). There was no correlation between 5'NT activity and the percentage of T4- or T8-positive lymphocytes or the T4/T8 ratio. Moreover, 5'NT activity was significantly decreased in both OKT4 (P < .025) and OKT8 (P < .05) enriched lymphocytes in homosexuals compared to controls. The data suggest that decreases in 5'NT may be a generalized defect of the peripheral blood T lymphocytes from active homosexuals that is independent of increases or decreases in specific T subpopulations or clinical status. It may contribute to the pathogenesis of AIDS.

AB - 5' Nucleotidase (5'NT) is an ectoenzyme associated with the plasma membrane of most mammalian cells. Low 5'NT activity has been observed in peripheral blood lymphocytes from patients with immunodeficiency states. 5'NT activity was measured in null and T-enriched lymphocytes from asymptomatic homosexual men and from 20 men with various degrees of the acquired immune deficiency syndrome (AIDS). Asymptomatic homosexuals were self-referred because of their concern about AIDS and were not necessarily representative of homosexuals in the general population. Enzyme activity was significantly decreased in both null (7.0 ± 2.4 nmol/106 cells/h) and T-enriched (12.0 ± 6.0 nmol/106 cells/h) lymphocytes in homosexuals as compared to lymphocytes from aged-matched heterosexual male and female controls (null = 10.8 ± 6.5 and T = 22.3 ± 10.6, P < .0001 and .008, respectively). Decreased activity was present regardless of whether the patients were asymptomatic, had prodromal symptoms such as fever, lymph node enlargement, weight loss and diarrhea, or had opportunistic infections or Kaposi's sarcoma. Homosexuals had a significantly higher fraction of lymphocytes expressing the activation antigens T10 (20% ± 3.3%) and Ia (13% ± 2.9%) than controls (11% ± 1.8% and 5% ± 0.8%, respectively, P < .05). They also had a significantly lower fraction of OKT4-positive helper lymphocytes than controls (22% ± 3.4% v 35% ± 2.2%, P < .05). 5'NT activity in lymphocytes enriched for null cells from homosexuals correlated inversely with the percentage of Ia-positive lymphocytes (r = -.655; P < .02). There was no correlation between 5'NT activity and the percentage of T4- or T8-positive lymphocytes or the T4/T8 ratio. Moreover, 5'NT activity was significantly decreased in both OKT4 (P < .025) and OKT8 (P < .05) enriched lymphocytes in homosexuals compared to controls. The data suggest that decreases in 5'NT may be a generalized defect of the peripheral blood T lymphocytes from active homosexuals that is independent of increases or decreases in specific T subpopulations or clinical status. It may contribute to the pathogenesis of AIDS.

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