Decreased transcription of the sodium-phosphate transporter gene in the hypophosphatemic mouse

J. F. Collins, L. A. Scheving, F. K. Ghishan

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Recently, it has been hypothesized that the proximal tubular Na+-P(i) transporter may play a role in murine X-linked hypophosphatemic vitamin D- resistant rickets. In the present investigation, Western blot analysis of renal brush-border membrane proteins, utilizing polyclonal antisera raised against the mouse Na+-P(i) transporter, revealed a predominant band at 87 kDa in normal and hypophosphatemic (Hyp) mice. The intensity of this band was reduced in the Hyp mouse by 4.5-fold (Hyp/normal = 0.22 ± 0.04, n = 3, P < 0.05). Additionally, immunohistochemical analysis of kidney cortex in both mice localized the protein to the apical membrane of the proximal tubules. Relative transcription rates of the Na+-P(i) transporter gene in the normal and Hyp mouse were then investigated. Nuclear run-on assays showed a 51 ± 0.02% decreased rate of transcription of the Na+-P(i) transporter gene in the Hyp mice (n = 3). Thus abnormal transcriptional control of this gene in the Hyp mouse likely plays a role in X-linked hypophosphatemia.

Original languageEnglish (US)
Pages (from-to)F439-F448
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume269
Issue number3 38-3
DOIs
StatePublished - 1995

Keywords

  • Hyp mouse model
  • X-linked hypophosphatemia
  • transcription rate analysis

ASJC Scopus subject areas

  • Physiology

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