Deep Sequencing of RNA from Ancient Maize Kernels

Sarah L. Fordyce, Maria C. Ávila-Arcos, Morten Rasmussen, Enrico Cappellini, J. Alberto Romero-Navarro, Nathan Wales, David E. Alquezar-Planas, Steven Penfield, Terence A. Brown, Jean Philippe Vielle-Calzada, Rafael Montiel, Tina Jørgensen, Nancy Odegaard, Michael Jacobs, Bernardo Arriaza, Thomas F.G. Higham, Christopher Bronk Ramsey, Eske Willerslev, M. Thomas P. Gilbert

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The characterization of biomolecules from ancient samples can shed otherwise unobtainable insights into the past. Despite the fundamental role of transcriptomal change in evolution, the potential of ancient RNA remains unexploited - perhaps due to dogma associated with the fragility of RNA. We hypothesize that seeds offer a plausible refuge for long-term RNA survival, due to the fundamental role of RNA during seed germination. Using RNA-Seq on cDNA synthesized from nucleic acid extracts, we validate this hypothesis through demonstration of partial transcriptomal recovery from two sources of ancient maize kernels. The results suggest that ancient seed transcriptomics may offer a powerful new tool with which to study plant domestication.

Original languageEnglish (US)
Article numbere50961
JournalPloS one
Volume8
Issue number1
DOIs
StatePublished - Jan 17 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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