Defects in coatomer protein I (COPI) transport cause blood feeding-induced mortality in Yellow Fever mosquitoes

Jun Isoe, Jennifer Collins, Hemant Badgandi, W. Anthony Day, Roger Miesfeld

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Blood feeding by vector mosquitoes provides the entry point for disease pathogens and presents an acute metabolic challenge that must be overcome to complete the gonotrophic cycle. Based on recent data showing that coatomer protein I (COPI) vesicle transport is involved in cellular processes beyond Golgi-endoplasmic reticulum retrograde protein trafficking, we disrupted COPI functions in the Yellow Fever mosquito Aedes aegypti to interfere with blood meal digestion. Surprisingly, we found that decreased expression of the γCOPI coatomer protein led to 89% mortality in blood-fed mosquitoes by 72 h postfeeding compared with 0% mortality in control dsRNA-injected blood-fed mosquitoes and 3% mortality in γCOPI dsRNA-injected sugar-fed mosquitoes. Similar results were obtained using dsRNA directed against five other COPI coatomer subunits (α, β, β′, δ, and ζ). We also examined midgut tissues by EM, quantitated heme in fecal samples, and characterized feeding-induced protein expression in midgut, fat body, and ovary tissues of COPI-deficient mosquitoes. We found that COPI defects disrupt epithelial cell membrane integrity, stimulate premature blood meal excretion, and block induced expression of several midgut protease genes. To study the role of COPI transport in ovarian development, we injected γCOPI dsRNA after blood feeding and found that, although blood digestion was normal, follicles in these mosquitoes were significantly smaller by 48 h postinjection and lacked eggshell proteins. Together, these data show that COPI functions are critical to mosquito blood digestion and egg maturation, a finding that could also apply to other blood-feeding arthropod vectors.

Original languageEnglish (US)
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number24
DOIs
StatePublished - Jun 14 2011

Fingerprint

Coatomer Protein
Yellow Fever
Protein Transport
Culicidae
Mortality
Digestion
Meals
Arthropod Vectors
Transport Vesicles
Fat Body
Aedes
Heme
Endoplasmic Reticulum

Keywords

  • Dengue RNAi
  • Oocyte vitellogenin
  • Serine protease

ASJC Scopus subject areas

  • General

Cite this

Defects in coatomer protein I (COPI) transport cause blood feeding-induced mortality in Yellow Fever mosquitoes. / Isoe, Jun; Collins, Jennifer; Badgandi, Hemant; Day, W. Anthony; Miesfeld, Roger.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 24, 14.06.2011.

Research output: Contribution to journalArticle

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