Defining the limits: Protein aggregation and toxicity in vivo

William M. Holmes, Courtney L. Klaips, Tricia R. Serio

Research output: Contribution to journalReview article

17 Scopus citations

Abstract

The proper folding of proteins to their functional forms is essential to cellular homeostasis. Perhaps not surprisingly, cells have evolved multiple pathways, some overlapping and others complementary, to resolve mis-folded proteins when they arise, ranging from refolding through the action of molecular chaperones to elimination through regulated proteolytic mechanisms. These protein quality control pathways are sufficient, under normal conditions, to maintain a functioning proteome, but in response to diverse environmental, genetic and/or stochastic events, protein mis-folding exceeds the corrective capacity of these pathways, leading to the accumulation of aggregates and ultimately toxicity. Particularly devastating examples of these effects include certain neurodegenerative diseases, such as Huntington's Disease, which are associated with the expansion of polyglutamine tracks in proteins. In these cases, protein mis-folding and aggregation are clear contributors to pathogenesis, but uncovering the precise mechanistic links between the two events remains an area of active research. Studies in the yeast Saccharomyces cerevisiae and other model systems have uncovered previously unanticipated complexity in aggregation pathways, the contributions of protein quality control processes to them and the cellular perturbations that result from them. Together these studies suggest that aggregate interactions and localization, rather than their size, are the crucial considerations in understanding the molecular basis of toxicity.

Original languageEnglish (US)
Pages (from-to)294-303
Number of pages10
JournalCritical reviews in biochemistry and molecular biology
Volume49
Issue number4
DOIs
StatePublished - Jan 1 2014

Keywords

  • Amyloid
  • Chaperone
  • Mis-folding
  • PolyQ
  • Prion
  • Toxicity

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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