Deformation responses of a physically cross-linked high molecular weight elastin-like protein polymer

Xiaoyi Wu, Rory E. Sallach, Jeffrey M. Caves, Vincent P. Conticello, Elliot L. Chaikof

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Recombinant protein polymers were synthesized and examined under various loading conditions to assess the mechanical stability and deformation responses of physically cross-linked, hydrated, protein polymer networks designed as triblock copolymers with central elastomeric and flanking plastic-like blocks. Uniaxial stress-strain properties, creep and stress relaxation behavior, as well as the effect of various mechanical preconditioning protocols on these responses were characterized. Significantly, we demonstrate for the first time that ABA triblock protein copolymers when redesigned with substantially larger endblock segments can withstand significantly greater loads. Furthermore, the presence of three distinct phases of deformation behavior was revealed upon subjecting physically cross-linked protein networks to step and cyclic loading protocols in which the magnitude of the imposed stress was incrementally increased over time. We speculate that these phases correspond to the stretch of polypeptide bonds, the conformational changes of polypeptide chains, and the disruption of physical cross-links. The capacity to select a genetically engineered protein polymer that is suitable for its intended application requires an appreciation of its viscoelastic characteristics and the capacity of both molecular structure and conditioning protocols to influence these properties.

Original languageEnglish (US)
Pages (from-to)1787-1794
Number of pages8
JournalBiomacromolecules
Volume9
Issue number7
DOIs
StatePublished - Jul 2008
Externally publishedYes

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Elastin
Polymers
Molecular Weight
Molecular weight
Proteins
Polypeptides
Recombinant proteins
Peptides
Mechanical stability
Stress relaxation
Molecular Structure
Recombinant Proteins
Molecular structure
Plastics
Block copolymers
Creep
Copolymers

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Deformation responses of a physically cross-linked high molecular weight elastin-like protein polymer. / Wu, Xiaoyi; Sallach, Rory E.; Caves, Jeffrey M.; Conticello, Vincent P.; Chaikof, Elliot L.

In: Biomacromolecules, Vol. 9, No. 7, 07.2008, p. 1787-1794.

Research output: Contribution to journalArticle

Wu, Xiaoyi ; Sallach, Rory E. ; Caves, Jeffrey M. ; Conticello, Vincent P. ; Chaikof, Elliot L. / Deformation responses of a physically cross-linked high molecular weight elastin-like protein polymer. In: Biomacromolecules. 2008 ; Vol. 9, No. 7. pp. 1787-1794.
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