Degradability of Soybean Meal Protein Fractions as Determined by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis

D. Romagnolo, C. E. Polan, W. E. Barbeau

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Degradation of soy protein subfractions was determined by suspending dacron bags containing soybean meal into the rumen from 0 to 72 h. Rumen-cannulated lactating Holstein cows consuming a diet of corn silage, alfalfa haylage, soybean meal, and high moisture shelled corn were used. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to monitor the rates of degradation in the rumen of protein subfractions. Fractional changes with time in the rumen were quantified using densitometric analysis of Coomassie blue stained protein bands. The β-conglycinin was more susceptible to rumen degradation than glycinin subunits. Acidic subunits (B1) of glycinin were degraded faster (.144 h–1) than were basic (B2) subfractions (.104 h–1). Estimates of B subfractional components and rates of degradation were used to predict degradability of soybean meal protein (73.8%). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and densitometric techniques can be used for measuring subfractional degradability in the rumen.

Original languageEnglish (US)
Pages (from-to)2379-2385
Number of pages7
JournalJournal of dairy science
Volume73
Issue number9
DOIs
StatePublished - 1990
Externally publishedYes

Keywords

  • degradation rate
  • electrophoresis
  • protein degradability

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

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